ME2 Antibody (F-5): sc-514850

ME2 Antibody (F-5) is a mouse monoclonal IgG1 antibody that detects ME2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ME2 (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ME2, also known as malic enzyme 2, is a crucial mitochondrial enzyme that plays a significant role in cellular metabolism by catalyzing the conversion of (s)-malate to pyruvate, a key step in the anaplerotic pathway. This enzymatic function is vital for maintaining the balance of metabolic intermediates, which is essential for energy production and biosynthesis in cells. Notably, ME2 exists as a homotetramer, and ME2 activity is intricately linked to the malate-aspartate shuttle, which is critical for the transport of reducing equivalents across the mitochondrial membrane. Dysregulation of ME2 has been implicated in various neuropsychiatric disorders, including schizophrenia and bipolar disorder, highlighting ME2 importance in neuronal function and neurotransmitter synthesis. ME2 interaction with ion channels in the brain further underscores ME2 relevance in the pathophysiology of epilepsy, making ME2 a significant target for research in both metabolic and neurological contexts.

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ME2 Antibody (F-5) References:

1. Sacred disease secrets revealed: the genetics of human epilepsy.  |  Turnbull, J., et al. 2005. Hum Mol Genet. 14: 2491-500. PMID: 16049035
2. Association analysis of malic enzyme 2 gene polymorphisms with idiopathic generalized epilepsy.  |  Lenzen, KP., et al. 2005. Epilepsia. 46: 1637-41. PMID: 16190936
3. Sacred disease secrets revealed: the genetics of human epilepsy.  |  Turnbull, J., et al. 2005. Hum Mol Genet. 14 Spec No. 2: 2491-2500. PMID: 16278970
4. Genetics of idiopathic generalized epilepsies.  |  Gardiner, M. 2005. Epilepsia. 46 Suppl 9: 15-20. PMID: 16302872
5. Cytosolic and mitochondrial malic enzyme isoforms differentially control insulin secretion.  |  Pongratz, RL., et al. 2007. J Biol Chem. 282: 200-7. PMID: 17102138
6. Malic enzyme 2 and susceptibility to psychosis and mania.  |  Lee, BD., et al. 2007. Psychiatry Res. 150: 1-11. PMID: 17258816
7. Arabidopsis NAD-malic enzyme functions as a homodimer and heterodimer and has a major impact on nocturnal metabolism.  |  Tronconi, MA., et al. 2008. Plant Physiol. 146: 1540-52. PMID: 18223148
8. Human malic enzyme-2 polymorphism in the GDR.  |  Stöhlmacher, P. and Haferland, W. 1989. Hum Hered. 39: 58-60. PMID: 2759628
9. SIRT5-mediated ME2 desuccinylation promotes cancer growth by enhancing mitochondrial respiration.  |  Teng, P., et al. 2024. Cell Death Differ. 31: 65-77. PMID: 38007551
10. AKT1 phosphorylation of cytoplasmic ME2 induces a metabolic switch to glycolysis for tumorigenesis.  |  Chen, T., et al. 2024. Nat Commun. 15: 686. PMID: 38263319
11. PRMT1-mediated methylation of ME2 promotes hepatocellular carcinoma growth by inhibiting ubiquitination.  |  Zhang, S., et al. 2024. Cell Death Dis. 15: 814. PMID: 39528487
12. ACAT1-Mediated ME2 Acetylation Drives Chemoresistance in Ovarian Cancer by Linking Glutaminolysis to Lactate Production.  |  Zheng, C., et al. 2025. Adv Sci (Weinh). e2416467. PMID: 39951294

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ME2 Antibody (F-5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-ME2 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ME2 Antibody (F-5) Specifications

Alternate Product Name: ME2

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 43-90

Epitope Target: ME2

Epitope Species: human

Product Reactivity/Detection: ME2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for ME2 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin