MDR1/ABCB1 Double Nickase Plasmid (h): sc-400178-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MDR1/ABCB1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MDR1/ABCB1 Double Nickase Plasmid (h) and MDR1/ABCB1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ABCB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MDR1/ABCB1 Antibody (D-11): sc-55510
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MDR1/ABCB1 Double Nickase Plasmid (h) | sc-400178-NIC | 20 µg | $410.00 | |||
MDR1/ABCB1 Double Nickase Plasmid (h2) | sc-400178-NIC-2 | 20 µg | $410.00 |
ABCB1 (MDR1/P-glycoprotein) encodes an ATP-binding cassette efflux transporter localized predominantly to the plasma membrane, where it uses ATP hydrolysis to export a broad range of xenobiotics and endogenous metabolites. MDR1/ABCB1 contributes to epithelial barrier and tissue-protective functions in organs such as intestine, liver, kidney, and blood–brain barrier, shaping intracellular drug disposition and cellular stress responses. Its activity intersects with detoxification and pharmacokinetic pathways, including coordinated regulation with nuclear receptor signaling (e.g., PXR, CAR) and membrane trafficking processes that control transporter abundance at the cell surface. Dysregulated ABCB1 expression or function is frequently studied in the context of multidrug resistance phenotypes in cancer models and in variability of drug response across tissues.
MDR1/ABCB1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ABCB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ABCB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ABCB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ABCB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.