Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MDH2 Double Nickase Plasmid (h): sc-402825-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MDH2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MDH2 Double Nickase Plasmid (h) and MDH2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MDH2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MDH2 Antibody (1G12): sc-293474
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MDH2 Double Nickase Plasmid (h) | sc-402825-NIC | 20 µg | $410.00 | |||
MDH2 Double Nickase Plasmid (h2) | sc-402825-NIC-2 | 20 µg | $410.00 |
MDH2 encodes mitochondrial malate dehydrogenase, a NAD-dependent enzyme that catalyzes the reversible conversion of malate to oxaloacetate, supporting the tricarboxylic acid (TCA) cycle and mitochondrial redox homeostasis. Through its role in oxidative metabolism, MDH2 contributes to ATP production, NADH/NAD⁺ balance, and anaplerotic flux that links mitochondrial function to broader carbon metabolism. Perturbation of MDH2 activity can alter respiratory capacity and metabolic signaling, processes frequently interrogated in studies of tumor metabolism, mitochondrial disorders, and neurodegeneration. As a core mitochondrial matrix enzyme, MDH2 is also relevant for investigating metabolic adaptation to hypoxia, nutrient stress, and reactive oxygen species dynamics.
MDH2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MDH2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MDH2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MDH2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MDH2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.