MDFIC CRISPR/Cas9 KO Plasmid (m): sc-421259
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MDFIC CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MDFIC genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MDFIC CRISPR/Cas9 KO Plasmid (m) | sc-421259 | 20 µg | $397.00 |
Overview
Mouse Mdfic encodes MDFIC, a MyoD family inhibitor–like nuclear protein implicated in transcriptional regulation and modulation of lineage-specific gene expression programs. MDFIC has been reported to influence bHLH transcription factor activity and is linked to control of cell differentiation states and proliferation-related transcriptional outputs. Through these regulatory roles, MDFIC can intersect with pathways governing developmental gene networks and context-dependent stress or growth responses. Altered MDFIC expression or function has been associated in the literature with dysregulated transcriptional control relevant to cancer biology and developmental phenotypes, supporting its value as a mechanistic research target.
MDFIC CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mdfic gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mdfic together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mdfic open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MDFIC protein expression.
This CRISPR knockout system enables efficient generation of Mdfic-deficient cell models for investigation of MDFIC signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mdfic exon(s) critical for MDFIC function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mdfic genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MDFIC CRISPR/Cas9 KO Plasmid (m) and MDFIC CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mdfic locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MDFIC HDR Plasmid (m) and MDFIC HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mdfic homology arms to support homology-directed repair at defined Mdfic target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.