MDFIC Antibody (D-9): sc-515212
- MDFIC Antibody (D-9) is a mouse monoclonal IgG1 MDFIC antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 181-199 within an internal region of MDFIC of human origin
- MDFIC Antibody (D-9) is recommended for detection of MDFIC of human origin by WB, IP, IF and ELISA
- Anti-MDFIC Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MDFIC Antibody (D-9) for WB applications. These reagents are now offered in bundles with MDFIC Antibody (D-9) (see ordering information below).
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MDFIC Antibody (D-9) is a mouse monoclonal IgG1 antibody that detects MDFIC of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MDFIC (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MDFIC, also known as MyoD family inhibitor domain-containing protein or HIC, is a 355 amino acid protein that exists in two alternatively spliced isoforms, p40 and p32, which predominantly localize to the nucleolus and cytoplasm, respectively. This localization allows MDFIC to effectively modulate the expression of viral genomes, particularly by down-regulating the transcription of HIV-1 while up-regulating the expression of HTLV-1 (T-cell leukemia virus type I). Furthermore, MDFIC plays a significant role in regulating the levels of β-catenin within the cell, which is vital for the proper functioning of the Wnt signaling pathway, a key regulator of cell proliferation and differentiation. The gene encoding MDFIC is located on human chromosome 7, a region associated with various genetic disorders, including Osteogenesis imperfecta and Williams-Beuren syndrome, highlighting the importance of this protein in both viral regulation and cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MDFIC Antibody (D-9) References:
- Molecular cloning of a novel human I-mfa domain-containing protein that differently regulates human T-cell leukemia virus type I and HIV-1 expression. | Thébault, S., et al. 2000. J Biol Chem. 275: 4848-57. PMID: 10671520
- Sequence requirement for the nucleolar localization of human I-mfa domain-containing protein (HIC p40). | Thébault, S., et al. 2000. Eur J Cell Biol. 79: 834-8. PMID: 11139147
- I-mfa domain proteins interact with Axin and affect its regulation of the Wnt and c-Jun N-terminal kinase signaling pathways. | Kusano, S. and Raab-Traub, N. 2002. Mol Cell Biol. 22: 6393-405. PMID: 12192039
- The human I-mfa domain-containing protein, HIC, interacts with cyclin T1 and modulates P-TEFb-dependent transcription. | Young, TM., et al. 2003. Mol Cell Biol. 23: 6373-84. PMID: 12944466
- Direct interaction of the human I-mfa domain-containing protein, HIC, with HIV-1 Tat results in cytoplasmic sequestration and control of Tat activity. | Gautier, VW., et al. 2005. Proc Natl Acad Sci U S A. 102: 16362-7. PMID: 16260749
- HIC gene, a candidate suppressor gene within a minimal region of loss at 7q31.1 in myeloid neoplasms. | Cigognini, D., et al. 2007. Leuk Res. 31: 477-82. PMID: 17064770
- Developmental regulators containing the I-mfa domain interact with T cyclins and Tat and modulate transcription. | Wang, Q., et al. 2007. J Mol Biol. 367: 630-46. PMID: 17289077
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MDFIC Antibody (D-9) | sc-515212 | 200 µg/ml | $322.00 | |||
MDFIC Antibody (D-9): m-IgG Fc BP-HRP Bundle | sc-528003 | 200 µg Ab; 10 µg BP | $361.00 | |||
MDFIC Antibody (D-9): m-IgG1 BP-HRP Bundle | sc-533376 | 200 µg Ab; 20 µg BP | $361.00 | |||
MDFIC Antibody (D-9) AC | sc-515212 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MDFIC Antibody (D-9) HRP | sc-515212 HRP | 200 µg/ml | $322.00 | |||
MDFIC Antibody (D-9) FITC | sc-515212 FITC | 200 µg/ml | $336.00 | |||
MDFIC Antibody (D-9) PE | sc-515212 PE | 200 µg/ml | $349.00 | |||
MDFIC Antibody (D-9) Alexa Fluor® 488 | sc-515212 AF488 | 200 µg/ml | $364.00 | |||
MDFIC Antibody (D-9) Alexa Fluor® 546 | sc-515212 AF546 | 200 µg/ml | $364.00 | |||
MDFIC Antibody (D-9) Alexa Fluor® 594 | sc-515212 AF594 | 200 µg/ml | $364.00 | |||
MDFIC Antibody (D-9) Alexa Fluor® 647 | sc-515212 AF647 | 200 µg/ml | $364.00 | |||
MDFIC Antibody (D-9) Alexa Fluor® 680 | sc-515212 AF680 | 200 µg/ml | $364.00 | |||
MDFIC Antibody (D-9) Alexa Fluor® 790 | sc-515212 AF790 | 200 µg/ml | $364.00 | |||
MDFIC (D-9) Neutralizing Peptide | sc-515212 P | 100 µg/0.5 ml | $69.00 |