MCT12 CRISPR/Cas9 KO Plasmid (h): sc-407365
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MCT12 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MCT12 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCT12 CRISPR/Cas9 KO Plasmid (h) | sc-407365 | 20 µg | $397.00 |
Overview
SLC16A12 encodes monocarboxylate transporter 12 (MCT12), a membrane transporter in the solute carrier 16 family implicated in transmembrane movement of small organic acids and related metabolites that contribute to cellular metabolic homeostasis. By influencing substrate availability across membranes, MCT12 can intersect with pathways governing lactate/pyruvate handling, redox balance, and metabolic coupling between cellular compartments. Altered SLC16A12 function has been associated with ocular phenotypes including cataract and changes in lens physiology, supporting its relevance to studies of epithelial transport, metabolite flux, and tissue-specific metabolic stress. These features make SLC16A12 a useful target for dissecting how monocarboxylate transport integrates with cellular energy metabolism and barrier/epithelial functions.
MCT12 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC16A12 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC16A12 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC16A12 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MCT12 protein expression.
This CRISPR knockout system enables efficient generation of SLC16A12-deficient cell models for investigation of MCT12 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC16A12 exon(s) critical for MCT12 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC16A12 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MCT12 CRISPR/Cas9 KO Plasmid (h) and MCT12 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC16A12 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MCT12 HDR Plasmid (h) and MCT12 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC16A12 homology arms to support homology-directed repair at defined SLC16A12 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.