MCPyV large T-antigen Antibody (CM2B4): sc-136172

MCPyV large T-antigen Antibody (CM2B4) is a mouse monoclonal IgG2b kappa light chain antibody that detects MCPyV large T-antigen protein of Merkel Cell Polyomavirus origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-MCPyV large T-antigen antibody (CM2B4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MCPyV large T-antigen plays a crucial role in viral life cycle and oncogenesis through regulation of cell cycle progression and inhibition of tumor suppressor proteins, such as retinoblastoma protein (Rb). By binding to Rb, MCPyV large T-antigen disrupts function, leading to uncontrolled cell proliferation, which is a hallmark of cancer development. This mechanism is particularly significant in Merkel cell carcinoma, where MCPyV large T-antigen serves as a specific marker for tumor cells, highlighting importance in both diagnosis and potential therapeutic targeting. Full-length MCPyV large T-antigen is a 125 kDa nuclear protein, but tumor-derived variants often exhibit truncating mutations, resulting in smaller isoforms. MCPyV large T-antigen monoclonal antibody (CM2B4) was raised against a peptide in exon 2 of T antigen locus, ensuring high specificity for MCPyV large T and 57kT isoforms while not detecting MCPyV small T antigen. Given widespread nature of human MCPyV infection, detection of MCPyV large T-antigen is vital for identifying Merkel cell tumors, making MCPyV large T-antigen (CM2B4) antibody an essential tool for researchers and clinicians in oncology.

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MCPyV large T-antigen Antibody (CM2B4) References:

1. Clonal integration of a polyomavirus in human Merkel cell carcinoma.  |  Feng, H., et al. 2008. Science. 319: 1096-100. PMID: 18202256
2. T antigen mutations are a human tumor-specific signature for Merkel cell polyomavirus.  |  Shuda, M., et al. 2008. Proc Natl Acad Sci U S A. 105: 16272-7. PMID: 18812503
3. Merkel cell polyomavirus in respiratory tract secretions.  |  Goh, S., et al. 2009. Emerg Infect Dis. 15: 489-91. PMID: 19239773
4. Human Merkel cell polyomavirus infection I. MCV T antigen expression in Merkel cell carcinoma, lymphoid tissues and lymphoid tumors.  |  Shuda, M., et al. 2009. Int J Cancer. 125: 1243-9. PMID: 19499546
5. Human Merkel cell polyomavirus infection II. MCV is a common human infection that can be detected by conformational capsid epitope immunoassays.  |  Tolstov, YL., et al. 2009. Int J Cancer. 125: 1250-6. PMID: 19499548
6. Merkel cell carcinoma: recent progress and current priorities on etiology, pathogenesis, and clinical management.  |  ,. 2009. J Clin Oncol. 27: 4021-6. PMID: 19597021
7. Merkel cell polyomavirus DNA detection in lesional and nonlesional skin from patients with Merkel cell carcinoma or other skin diseases.  |  Foulongne, V., et al. 2010. Br J Dermatol. 162: 59-63. PMID: 19678822
8. Merkel cell polyomavirus DNA in persons without merkel cell carcinoma.  |  Wieland, U., et al. 2009. Emerg Infect Dis. 15: 1496-8. PMID: 19788824
9. Merkel cell polyomavirus: a specific marker for Merkel cell carcinoma in histologically similar tumors.  |  Duncavage, EJ., et al. 2009. Am J Surg Pathol. 33: 1771-7. PMID: 19809278
10. Significant overexpression of the Merkel cell polyomavirus (MCPyV) large T antigen in Merkel cell carcinoma.  |  Erovic, BM., et al. 2013. Head Neck. 35: 184-9. PMID: 22307956
11. Expression of Merkelcell polyomavirus (MCPyV) large T-antigen in Merkel cell carcinoma lymph node metastases predicts poor outcome.  |  Haymerle, G., et al. 2017. PLoS One. 12: e0180426. PMID: 28763479
12. MCPyV Large T Antigen-Induced Atonal Homolog 1 Is a Lineage-Dependency Oncogene in Merkel Cell Carcinoma.  |  Fan, K., et al. 2020. J Invest Dermatol. 140: 56-65.e3. PMID: 31283928

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MCPyV large T-antigen Antibody (CM2B4) has 186 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MCPyV large T-antigen Antibody (CM2B4) Specifications

Alternate Product Name: Merkel Cell Polyomavirus Tumor Antigen (Large)

Product Clone Name: CM2B4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: Merkel Cell Polyomavirus

Epitope Species: Merkel Cell Polyomavirus

Product Reactivity/Detection: Merkel Cell Polyomavirus large T antigen

Species Reactivity/Detection: Merkel Cell Polyomavirus

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 186 citations in various scientific publications

Available Conjugates/Forms for MCPyV large T-antigen Antibody (CM2B4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin