Anti-MCPyV large T-antigen Antibody (CM2B4) recommended for detection of Merkel Cell Polyomavirus by WB, IP, IF and IHC(P)

Anti-MCPyV large T-antigen Antibody (CM2B4): sc-136172

Anti-MCPyV large T-antigen Antibody (CM2B4) is rated 4.2 out of 5 by 9.
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Datasheets

    • Anti-MCPyV large T-antigen Antibody (CM2B4) is a mouse monoclonal IgG2b κ MCPyV large T-antigen antibody, cited in 165 publications, provided at 200 µg/ml
    • raised against large T/57kT exon 2 peptides of Merkel cell polyomavirus
    • MCPyV large T-antigen Antibody (CM2B4) is recommended for detection of Merkel Cell Polyomavirus by WB, IP, IF and IHC(P)
    • Anti-MCPyV large T-antigen Antibody (CM2B4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MCPyV large T-antigen (CM2B4): sc-136172.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for MCPyV large T-antigen Antibody (CM2B4) for WB and IHC(P) applications. This reagent is now offered in a bundle with MCPyV large T-antigen Antibody (CM2B4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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How many tests does the sample of MCPYV do? Is this a concentrate or RTU?

Asked by: Kirish34
Thank you for your question. The antibody sample is provided as 10 ug in 50 ul. This is usually sufficient for 1-3 tests of the primary antibody, enough to determine if it will work for your purpose before purchasing a full size vial for further optimization.
Answered by: Technical Support
Date published: 2019-01-26

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2019-01-11
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Rated 5 out of 5 by from Fantastic antibody! My lab has been using this antibody for the last several years. We are very happy with the quality of the antibody. Works really well for IF and WB.
Date published: 2021-02-14
Rated 5 out of 5 by from works very well and reproducible perfect antibody for the detection of Merkel cell polyomavirus Large T angtigen by Western or immunohistochemistry
Date published: 2020-10-13
Rated 5 out of 5 by from CM2B4 CM2B4 gave a nice signal in about 60% of our tumor samples (MCC)
Date published: 2018-03-23
Rated 5 out of 5 by from Great This antibody is very good for IF staining. The background is very low.
Date published: 2017-08-15
Rated 5 out of 5 by from Published results in IHC Works very well for IHC purposes. I've had this antibody for over a year and it still works amazingly well.
Date published: 2017-02-08
Rated 4 out of 5 by from Well published Established monoclonal antibody for MCPyV large T-antigen, well published for Western Blot and IF.
Date published: 2016-12-22
Rated 3 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-06-19
Rated 1 out of 5 by from Obtained false positive results in IF Obtained false positive results in IF.
Date published: 2015-06-16
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