raised against large T/57kT exon 2 peptides of Merkel cell polyomavirus
MCPyV large T-antigen Antibody (CM2B4) is recommended for detection of Merkel Cell Polyomavirus by WB, IP, IF and IHC(P)
Anti-MCPyV large T-antigen Antibody (CM2B4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MCPyV large T-antigen (CM2B4): sc-136172.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
MCPyV large T-antigen Antibody (CM2B4) is a high quality monoclonal MCPyV large T-antigen antibody (also designated MCPyV large T-antigen antibody) suitable for the detection of the MCPyV large T-antigen protein of Merkel Cell Polyomavirus origin. MCPyV large T-antigen Antibody (CM2B4) is available as both the non-conjugated anti-MCPyV large T-antigen antibody form, as well as multiple conjugated forms of anti-MCPyV large T-antigen antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Merkel cells are round neuroendocrine cells found in skin that have synaptic contacts with somatosensory afferents. Responsible for touch and pressure sensation, Merkel cells can turn malignant and form a rare but aggressive form of skin cancer known as Merkel cell carcinoma (MCC). Approximately 80% of MCC are caused by a newly-described polyomavirus called Merkel cell polyomavirus, also known as MCPyV or MCV, that expresses a large T antigen in tumor cells. Full-length MCPyV large T antigen is a 125 kDa nuclear protein but MCPyV T antigens obtained from tumors have natural truncating mutations resulting in variably-sized, smaller proteins. MCPyV large T-antigen (CM2B4) was raised against a peptide in exon 2 of the T antigen locus and is highly specific for MCPyV large T and 57kT isoforms but will not detect MCPyV small T antigen. While human MCPyV infection is widespread, MCPyV large T antigen is a specific marker for Merkel cell tumors.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
How many tests does the sample of MCPYV do? Is this a concentrate or RTU?
Asked by: Kirish34
Thank you for your question. The antibody sample is provided as 10 ug in 50 ul. This is usually sufficient for 1-3 tests of the primary antibody, enough to determine if it will work for your purpose before purchasing a full size vial for further optimization.
Answered by: Technical Support
Date published: 2019-01-26
What is the recommended secondary reagent to be used with this mouse monoclonal primary?
Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here:
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn