MCP-1 Antibody (ECE.2) recommended for detection of murine MCP-1 of mouse origin by WB, IP and IF

MCP-1 Antibody (ECE.2): sc-52701

MCP-1 Antibody (ECE.2) is rated 3.9 out of 5 by 9.
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Datasheets

    • MCP-1 Antibody (ECE.2) is a rat monoclonal IgG1 provided at 100 µg/ml
    • raised against MCP-1 of mouse origin
    • recommended for detection of murine MCP-1 of mouse origin by WB, IP and IF
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MCP-1 (ECE.2): sc-52701.

Dear Sir/Madam, Which secondary antibody can I use? Because I didn't find secondary antibody for rat IgG1. In your company just this antibody can use for checking mouse MCP-1. Thank you very much!

Asked by: ruozhi
Thank you for your question. MCP-1 Antibody (ECE.2) is a rat monoclonal IgG1. We recommend using any secondary antibody that is anti-rat IgG. Unfortunately, we do not currently offer any secondary antibodies anti-rat IgG at this time. Please check back for updates in the future!
Answered by: Technical Support
Date published: 2019-08-17

I am using MCP-1 (ECE.2): sc-52701 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from the antibody works great! I bought it for our mice aorta tissue staining, it works very well.
Date published: 2019-02-05
Rated 3 out of 5 by from WB 2C12 secreted medium It doesn't work in the conditioned medium of C2C12 cells in Westren blotting at the diluition of 1:1000
Date published: 2018-10-05
Rated 4 out of 5 by from Good antibody This antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 4 out of 5 by from 一般吧 it is a good antibody suitable for westernblot 一般般 这个不好孵
Date published: 2017-08-30
Rated 5 out of 5 by from good antibody Very small MW only 12kda, I recommend use 0.22um PVDF and expose long time and loading more than 60ug protein,1:200 antibody been diluted.
Date published: 2017-07-01
Rated 5 out of 5 by from specific target band The specificity of MCP-1 antibody is very good,i have a good specific band use dilution 1:500.
Date published: 2017-05-27
Rated 5 out of 5 by from clear band seen We observed clear band when diluted 1:500 ,Great help to us, strongly recommended for mouse brain tissue detection..
Date published: 2017-05-23
Rated 2 out of 5 by from no bands in THP-1 WB signal can not be obtained using this antibody sample at 1:500 dilultion against THP-1 whole cell lysates. Good and quick service. Thanks.
Date published: 2017-05-19
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