Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MCM3 Double Nickase Plasmid (h): sc-402076-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MCM3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MCM3 Double Nickase Plasmid (h) and MCM3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MCM3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MCM3 Antibody (E-8): sc-390480
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCM3 Double Nickase Plasmid (h) | sc-402076-NIC | 20 µg | $410.00 | |||
MCM3 Double Nickase Plasmid (h2) | sc-402076-NIC-2 | 20 µg | $410.00 |
MCM3 encodes minichromosome maintenance complex component 3, a core subunit of the MCM2–7 helicase required for origin licensing and DNA replication fork progression during S phase. As part of the pre-replication complex, MCM3 coordinates with CDC45 and GINS to drive replisome assembly, supporting genome stability and faithful chromosome duplication. Perturbation of MCM3-dependent replication dynamics can promote replication stress, altered cell-cycle checkpoints, and accumulation of DNA damage, processes frequently investigated in the context of cancer biology and proliferative disorders. MCM3 is therefore widely used as a mechanistic entry point to study DNA replication control, replication stress responses, and cell-cycle regulation.
MCM3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MCM3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MCM3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MCM3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MCM3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.