Mcl-1 Antibody (MCL-401): sc-69839

Mcl-1 Antibody (MCL-401) is a mouse monoclonal IgG1 antibody raised against recombinant full-length human Mcl-1, designed for the detection of Mcl-1 in human samples via western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. Mcl-1, also called Myeloid cell leukemia-1, Mcl1, and Gm52627, is a critical member of the B-cell lymphoma 2 (Bcl-2) family of proteins that regulate apoptosis, functioning as an anti-apoptotic factor by sequestering pro-apoptotic proteins like Bak and Bax, thus preventing mitochondrial outer membrane permeabilization and subsequent cell death. The function of Mcl-1 is essential for cell survival and homeostasis, particularly in early hematopoietic progenitors, as Mcl-1 ensures the balance between cell proliferation and apoptosis. Mcl-1′s stability and expression levels are tightly regulated through various mechanisms, including phosphorylation by glycogen synthase kinase 3 (GSK3), which targets Mcl-1 for ubiquitination and proteasomal degradation, and transcriptional regulation by factors such as STAT3. The importance of Mcl-1 is underscored by Mcl-1′s involvement in cancer development and progression, where overexpression contributes to tumor survival, chemoresistance, and poor prognosis in various malignancies including leukemia and lymphoma. Understanding Mcl-1′s function and interactions is crucial for therapeutic interventions, as targeting Mcl-1 can restore apoptotic processes in cancer cells. Anti-Mcl-1 antibody (MCL-401) serves as a valuable tool for researchers investigating the molecular mechanisms of apoptosis, the role of Mcl-1 in cell survival, and implications in human diseases.

The preferred Mcl-1 antibody is Mcl-1 Antibody (22): sc-12756.