MCC Antibody (A-9): sc-398216
- MCC Antibody (A-9) is a mouse monoclonal IgG1 κ MCC antibody provided at 200 µg/ml
- raised against amino acids 661-945 mapping within an internal region of MCC of human origin
- MCC Antibody (A-9) is recommended for detection of MCC isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-MCC Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for MCC Antibody (A-9) for WB applications. This reagent is now offered in a bundle with MCC Antibody (A-9) (see ordering information below).
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MCC Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects MCC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MCC antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MCC protein, also known as MCC1, is a coiled-coil protein primarily localized in the cytoplasm and plays a crucial role in cell cycle regulation. MCC protein is involved in negatively regulating cell cycle progression during G1 to S phase transition by modulating NFκB signaling pathway. MCC protein interacts with NFκB inhibitor, IκBβ, which is essential for stabilization, thereby preventing nuclear translocation and signaling of NFκB. Such interaction suggests MCC protein may function as a tumor suppressor, as proper regulation is vital for maintaining normal cell cycle dynamics. MCC protein undergoes phosphorylation, and phosphorylation state varies throughout cell cycle, indicating activity may be tightly controlled by post-translational modifications. MCC protein is highly phosphorylated during G1 to S phase transition, while exhibiting lower phosphorylation levels in G0/G1 phase. Overexpression of MCC protein has been shown to decrease cells entering S phase, further underscoring significance in cell cycle control and potential implications in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MCC Antibody (A-9) References:
- [The possible role of loss of heterozygosity at APC, MCC and DCC genetic loci in esophageal carcinoma]. | Wang, M., et al. 1999. Zhonghua Zhong Liu Za Zhi. 21: 16-8. PMID: 11776787
- Telomere erosion is independent of microsatellite instability but related to loss of heterozygosity in gastric cancer. | Fang, DC., et al. 2001. World J Gastroenterol. 7: 522-6. PMID: 11819821
- Study of loss of heterozygosity at DCC and APC/MCC genetic loci of gastric cancer. | Wang, D., et al. 1999. Chin Med Sci J. 14: 107-11. PMID: 12901620
- Loss of heterozygosity at APC and MCC genes of oral cancer and leukoplakia tissues from Indian tobacco chewers. | Sikdar, N., et al. 2003. J Oral Pathol Med. 32: 450-4. PMID: 12901725
- A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. | Bouwmeester, T., et al. 2004. Nat Cell Biol. 6: 97-105. PMID: 14743216
- [Study on loss of heterozygosity and microsatellite instability in hepatocellular carcinoma]. | Cong, WM., et al. 2005. Zhonghua Bing Li Xue Za Zhi. 34: 71-4. PMID: 15842799
- Promoter methylation of the mutated in colorectal cancer gene is a frequent early event in colorectal cancer. | Kohonen-Corish, MR., et al. 2007. Oncogene. 26: 4435-41. PMID: 17260021
- Identification of a gene located at chromosome 5q21 that is mutated in colorectal cancers. | Kinzler, KW., et al. 1991. Science. 251: 1366-70. PMID: 1848370
- MCC, a cytoplasmic protein that blocks cell cycle progression from the G0/G1 to S phase. | Matsumine, A., et al. 1996. J Biol Chem. 271: 10341-6. PMID: 8626604
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCC Antibody (A-9) | sc-398216 | 200 µg/ml | $322.00 | |||
MCC Antibody (A-9): m-IgG1 BP-HRP Bundle | sc-531765 | 200 µg Ab; 20 µg BP | $361.00 | |||
MCC Antibody (A-9) AC | sc-398216 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MCC Antibody (A-9) HRP | sc-398216 HRP | 200 µg/ml | $322.00 | |||
MCC Antibody (A-9) FITC | sc-398216 FITC | 200 µg/ml | $336.00 | |||
MCC Antibody (A-9) PE | sc-398216 PE | 200 µg/ml | $349.00 | |||
MCC Antibody (A-9) Alexa Fluor® 488 | sc-398216 AF488 | 200 µg/ml | $364.00 | |||
MCC Antibody (A-9) Alexa Fluor® 546 | sc-398216 AF546 | 200 µg/ml | $364.00 | |||
MCC Antibody (A-9) Alexa Fluor® 594 | sc-398216 AF594 | 200 µg/ml | $364.00 | |||
MCC Antibody (A-9) Alexa Fluor® 647 | sc-398216 AF647 | 200 µg/ml | $364.00 | |||
MCC Antibody (A-9) Alexa Fluor® 680 | sc-398216 AF680 | 200 µg/ml | $364.00 | |||
MCC Antibody (A-9) Alexa Fluor® 790 | sc-398216 AF790 | 200 µg/ml | $364.00 |