Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MC1-R Double Nickase Plasmid (h): sc-402152-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MC1-R Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MC1-R Double Nickase Plasmid (h) and MC1-R Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MC1R. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MC1-R Antibody (578CT6.2.3): sc-517344
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MC1-R Double Nickase Plasmid (h) | sc-402152-NIC | 20 µg | $410.00 | |||
MC1-R Double Nickase Plasmid (h2) | sc-402152-NIC-2 | 20 µg | $410.00 |
MC1R encodes the melanocortin 1 receptor (MC1-R), a G protein–coupled receptor predominantly expressed in melanocytes that regulates pigmentation and cellular stress responses. Upon binding melanocortin peptides such as α-MSH, MC1-R activates Gs/adenylyl cyclase signaling to elevate cAMP, driving MITF-dependent transcription and shifting melanogenesis toward eumelanin production. This pathway integrates with UV-induced DNA damage responses and redox homeostasis, influencing melanocyte survival and inflammatory signaling in the skin. Genetic variation and altered MC1R activity are associated with pigmentation phenotypes and increased susceptibility to UV-related skin pathology, making it a widely used locus for mechanistic studies in melanocyte biology and photobiology.
MC1-R Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MC1R locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MC1R. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MC1R function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MC1R-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.