MBP Antibody (A-3): sc-376995

MBP Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects myelin basic protein (MBP) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MBP (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Myelin basic protein plays a crucial role as the major extrinsic membrane protein of central nervous system myelin, contributing significantly to the formation and maintenance of the myelin sheath that insulates nerve fibers. MBP functions vitally for rapid transmission of electrical signals in the nervous system, and phosphorylation at Threonine 125 serves as a key regulatory mechanism influencing myelin sheath stability. Mitogen-activated protein kinases modulate this phosphorylation during myelinogenesis and in demyelinating diseases such as multiple sclerosis. MBP phosphorylation responds to high-frequency stimulation of the alveus, the myelinated output fibers of the hippocampus, suggesting increased neuronal activity can alter MBP′s phosphorylation state through calcium-mediated activation of axonal nitric oxide synthase, which produces nitric oxide and superoxide as intercellular messengers. This intricate regulatory process underscores MBP′s importance in both normal and pathological states of the nervous system, making anti-MBP antibody (A-3) an essential tool for researchers studying myelin-related functions and diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MBP Antibody (A-3) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-MBP Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MBP Antibody (A-3) Specifications

Alternate Product Name: myelin basic protein

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 54-90

Epitope Target: MBP

Epitope Species: human

Product Reactivity/Detection: MBP isoforms 3 and 4 (also designated MBP1 and MBP2)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for MBP Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred MBP antibody is MBP Antibody (F-6): sc-271524.