MaxiKβ Antibody (A-5): sc-377023

MaxiKβ Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects MaxiKβ protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MaxiKβ antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The KCNMB1 gene, located on chromosome 5q35.1, encodes the 191 amino-acid MaxiKβ subunit 1, which is crucial for forming calcium-activated potassium channels. MaxiKβ subunit 1 features two putative transmembrane domains and an extracellular loop with three consensus sequences for N-linked glycosylation, as well as four cysteine residues that may form disulfide bridges, contributing to structural integrity and function. MaxiKβ subunit 1 is predominantly expressed in smooth muscle tissue but is also present in the brain, liver, and lymphatic tissues, where MaxiKβ subunit 1 plays a vital role in modulating MaxiKα subunit activity. The interaction between MaxiKβ and MaxiKα enhances channel sensitivity to calcium and voltage, making the α/β1 channel the most responsive of all Maxi channels to calcium levels. This sensitivity is essential for proper vasoregulation, as MaxiKβ allows for precise control of arterial relaxation, thereby influencing blood flow and pressure.

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MaxiKβ Antibody (A-5) References:

1. Molecular basis of fast inactivation in voltage and Ca2+-activated K+ channels: a transmembrane beta-subunit homolog.  |  Wallner, M., et al. 1999. Proc Natl Acad Sci U S A. 96: 4137-42. PMID: 10097176
2. Cloning and functional characterization of novel large conductance calcium-activated potassium channel beta subunits, hKCNMB3 and hKCNMB4.  |  Brenner, R., et al. 2000. J Biol Chem. 275: 6453-61. PMID: 10692449
3. Vasoregulation by the beta1 subunit of the calcium-activated potassium channel.  |  Brenner, R., et al. 2000. Nature. 407: 870-6. PMID: 11057658
4. Beta1-subunit of MaxiK channel in smooth muscle: a key molecule which tunes muscle mechanical activity.  |  Tanaka, Y., et al. 2004. J Pharmacol Sci. 94: 339-47. PMID: 15107573
5. MaxiK channel roles in blood vessel relaxations induced by endothelium-derived relaxing factors and their molecular mechanisms.  |  Tanaka, Y., et al. 2004. J Smooth Muscle Res. 40: 125-53. PMID: 15655302
6. MaxiK channel and cell signalling.  |  Toro, L., et al. 2014. Pflugers Arch. 466: 875-86. PMID: 24077696
7. MaxiK channel interactome reveals its interaction with GABA transporter 3 and heat shock protein 60 in the mammalian brain.  |  Singh, H., et al. 2016. Neuroscience. 317: 76-107. PMID: 26772433
8. Urothelial MaxiK-activity regulates mucosal and detrusor metabolism.  |  Wang, Y., et al. 2017. PLoS One. 12: e0189387. PMID: 29281667
9. Primary sequence and immunological characterization of beta-subunit of high conductance Ca(2+)-activated K+ channel from smooth muscle.  |  Knaus, HG., et al. 1994. J Biol Chem. 269: 17274-8. PMID: 8006036
10. Cloning, expression, and distribution of a Ca(2+)-activated K+ channel beta-subunit from human brain.  |  Tseng-Crank, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 9200-5. PMID: 8799178
11. Molecular constituents of maxi KCa channels in human coronary smooth muscle: predominant alpha + beta subunit complexes.  |  Tanaka, Y., et al. 1997. J Physiol. 502 (Pt 3): 545-57. PMID: 9279807
12. Human and rodent MaxiK channel beta-subunit genes: cloning and characterization.  |  Jiang, Z., et al. 1999. Genomics. 55: 57-67. PMID: 9888999

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MaxiKβ Antibody (A-5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-MaxiKβ Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MaxiKβ Antibody (A-5) Specifications

Alternate Product Name: MaxiKβ

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-191

Epitope Target: MaxiKβ

Epitope Species: human

Product Reactivity/Detection: MaxiKβ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for MaxiKβ Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin