Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAT IIα Double Nickase Plasmid (h): sc-402566-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAT IIα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MAT IIα Double Nickase Plasmid (h) and MAT IIα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAT2A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAT II Antibody (F-12): sc-398917
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAT IIα Double Nickase Plasmid (h) | sc-402566-NIC | 20 µg | $410.00 | |||
MAT IIα Double Nickase Plasmid (h2) | sc-402566-NIC-2 | 20 µg | $410.00 |
MAT2A encodes the catalytic α subunit of methionine adenosyltransferase II (MAT IIα), which synthesizes S-adenosyl-L-methionine (SAM), the principal methyl donor for DNA, RNA, and histone methylation reactions. By controlling intracellular SAM availability, MAT IIα links methionine cycle and one-carbon metabolism to epigenetic regulation, polyamine biosynthesis, and redox balance through transsulfuration. MAT2A activity is tightly coupled to proliferative programs and nutrient-sensing states, making it relevant to studies of metabolic adaptation and chromatin-dependent gene regulation. Altered MAT2A expression or dependency has been reported in multiple disease contexts, including cancer metabolism and liver-related pathophysiology, supporting its use as a molecular handle for interrogating methylation-driven phenotypes.
MAT IIα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAT2A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAT2A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAT2A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAT2A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.