Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAT Iα CRISPR/Cas9 KO Plasmid (m): sc-419102
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAT Iα CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MAT Iα genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAT Iα CRISPR/Cas9 KO Plasmid (m) | sc-419102 | 20 µg | $397.00 |
Overview
Mat1a encodes methionine adenosyltransferase I alpha (MAT Iα), the major hepatic enzyme that catalyzes synthesis of S-adenosyl-L-methionine (SAM), the principal methyl donor for DNA, RNA, protein, and lipid methylation reactions. Through control of SAM/SAH balance, MAT Iα links methionine metabolism to one-carbon metabolism, transsulfuration, and redox homeostasis, influencing epigenetic regulation and global methylation capacity. Altered MAT1A activity is associated with disrupted methylation-dependent gene expression programs and metabolic stress responses, making it relevant to studies of liver physiology, hepatocyte differentiation, and mechanisms of metabolic dysfunction.
MAT Iα CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mat1a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mat1a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mat1a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MAT Iα protein expression.
This CRISPR knockout system enables efficient generation of Mat1a-deficient cell models for investigation of MAT Iα signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mat1a exon(s) critical for MAT Iα function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mat1a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MAT Iα CRISPR/Cas9 KO Plasmid (m) and MAT Iα CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mat1a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MAT Iα HDR Plasmid (m) and MAT Iα HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mat1a homology arms to support homology-directed repair at defined Mat1a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.