MARK2 Double Nickase Plasmid (h): sc-403714-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MARK2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MARK2 Double Nickase Plasmid (h) and MARK2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MARK2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MARK2 Antibody (B-1): sc-365405
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MARK2 Double Nickase Plasmid (h) | sc-403714-NIC | 20 µg | $410.00 | |||
MARK2 Double Nickase Plasmid (h2) | sc-403714-NIC-2 | 20 µg | $410.00 |
Human MARK2 (microtubule affinity-regulating kinase 2) is a serine/threonine kinase of the PAR-1 family that phosphorylates microtubule-associated proteins, including tau, to regulate microtubule stability and cytoskeletal dynamics. It functions downstream of polarity signaling modules and contributes to establishment of apical–basal polarity, asymmetric cell division, and organization of the actin–microtubule network. MARK2 activity interfaces with cell-cycle control, neuronal differentiation, and stress-responsive signaling, linking it to processes such as axon specification and intracellular transport. Dysregulated MARK2 signaling has been associated with altered phosphorylation programs and polarity defects observed across neurodegeneration- and cancer-related research contexts.
MARK2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MARK2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MARK2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MARK2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MARK2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.