MARCKS (h2): 293T Lysate: sc-177518
- human MARCKS transfected 293T lysate
- 100 µg protein in 200 µl SDS-PAGE buffer; suitable as Western Blotting control for MARCKS antibodies
- Suitable control antibody: MARCKS Antibody (JK-8): sc-100777 is recommended as control antibody for monitoring of MARCKS expression knockdown by Western blotting or immunofluorescence
- should be stored at -20° C and repeated freezing and thawing should be minimized
- sample vial should be boiled once prior to use
- non-transfected control 293T lysate (sc-117752)
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MARCKS (h2): 293T Lysate, derived from the HEK 293T cell line, is crucial for researching the Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) protein, which plays a significant role in cellular signaling and cytoskeletal organization. The 293T cells, enhanced with the SV40 large T antigen for improved replication and protein expression, provide an ideal system for studying MARCKS' interactions and functions. Researchers use this lysate to delve into how MARCKS modulates actin dynamics and membrane-cytoskeleton interactions through its phosphorylation by Protein Kinase C (PKC) and its binding to calmodulin, pivotal in cellular processes such as migration and adhesion. Techniques such as co-immunoprecipitation, fluorescence microscopy, and kinase activity assays are employed to investigate the reversible phosphorylation of MARCKS, which regulates its interaction with actin and phospholipids. This research offers insights into the fundamental cellular mechanisms by which MARCKS influences signal transduction pathways and cellular architecture. The focus is strictly on understanding the biochemical properties and biological roles of MARCKS in cellular functions, exploring its potential as a key regulator in the basic science of cellular dynamics.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MARCKS (h2): 293T Lysate | sc-177518 | 100 µg/200 µl | $236.00 |