MARCH8 CRISPR/Cas9 KO Plasmid (h): sc-405928
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MARCH8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MARCH8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MARCH8 CRISPR/Cas9 KO Plasmid (h) | sc-405928 | 20 µg | $397.00 |
Overview
MARCH8 (membrane associated ring-CH-type finger 8) encodes an E3 ubiquitin ligase that localizes to endosomal and plasma membrane compartments and regulates the ubiquitination-dependent turnover and trafficking of multiple membrane proteins. By controlling endocytosis, lysosomal routing, and surface availability of receptors and transporters, MARCH8 influences antigen presentation, immune signaling, and membrane protein quality control pathways. Its activity has been linked to modulation of viral envelope glycoprotein incorporation and restriction of viral particle infectivity, highlighting roles at the interface of host defense and membrane biology. Dysregulated ubiquitin-mediated trafficking programs involving MARCH8 are relevant to studies of immune dysfunction and pathogen–host interactions in human cells.
MARCH8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MARCH8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MARCH8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MARCH8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MARCH8 protein expression.
This CRISPR knockout system enables efficient generation of MARCH8-deficient cell models for investigation of MARCH8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MARCH8 exon(s) critical for MARCH8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MARCH8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MARCH8 CRISPR/Cas9 KO Plasmid (h) and MARCH8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MARCH8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MARCH8 HDR Plasmid (h) and MARCH8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MARCH8 homology arms to support homology-directed repair at defined MARCH8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.