MARCH11 CRISPR/Cas9 KO Plasmid (h): sc-416041
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MARCH11 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MARCH11 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MARCH11 CRISPR/Cas9 KO Plasmid (h) | sc-416041 | 20 µg | $397.00 |
Overview
MARCH11 encodes a membrane-associated RING-CH (MARCH) family E3 ubiquitin ligase that localizes predominantly to endosomal and secretory membranes, where it promotes ubiquitination-dependent sorting of select transmembrane proteins. By regulating cargo trafficking through endocytosis and lysosomal degradation pathways, MARCH11 helps shape cell-surface protein composition and intracellular membrane dynamics. This activity links MARCH11 to broader ubiquitin–proteasome and endolysosomal network control, processes frequently implicated in immune regulation, cellular stress responses, and oncogenic signaling. Dysregulated ubiquitination and membrane protein turnover are also associated with altered receptor signaling and proteostasis defects relevant to cancer biology and other disease contexts.
MARCH11 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MARCH11 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MARCH11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MARCH11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MARCH11 protein expression.
This CRISPR knockout system enables efficient generation of MARCH11-deficient cell models for investigation of MARCH11 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MARCH11 exon(s) critical for MARCH11 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MARCH11 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MARCH11 CRISPR/Cas9 KO Plasmid (h) and MARCH11 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MARCH11 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MARCH11 HDR Plasmid (h) and MARCH11 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MARCH11 homology arms to support homology-directed repair at defined MARCH11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.