MAP1D Antibody (B-8): sc-515155
- MAP1D Antibody (B-8) is a mouse monoclonal IgG1 κ MAP1D antibody provided at 200 µg/ml
- raised against amino acids 188-253 mapping within an internal region of MAP1D of human origin
- MAP1D Antibody (B-8) is recommended for detection of MAP1D of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-MAP1D Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MAP1D Antibody (B-8) for WB applications. These reagents are now offered in bundles with MAP1D Antibody (B-8) (see ordering information below).
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MAP1D Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects MAP1D protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MAP1D antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAP1D, also known as methionyl aminopeptidase type 1D or METAP1D, is a 335 amino acid mitochondrial protein that plays a crucial role in protein maturation by removing methionine from the N-terminus of nascent proteins, a process essential for proper protein functioning. MAP1D′s enzymatic activity is particularly important in cellular stress responses and has been implicated in tumorigenesis, with elevated expression observed in colon cancer cell lines. MAP1D binds two cobalt ions per subunit, which is vital for enzymatic function, and is encoded by a gene located on human chromosome 2q31.1. This chromosome contains over 1,400 genes and is associated with various genetic diseases, including Harlequin ichthyosis and Alström syndrome. MAP1D′s interactions with other proteins and involvement in critical cellular processes make MAP1D a valuable target for research in cancer biology and mitochondrial function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MAP1D Antibody (B-8) References:
- Alstrom syndrome: confirmation of linkage to chromosome 2p12-13 and phenotypic heterogeneity in three affected sibs. | Zumsteg, U., et al. 2000. J Med Genet. 37: E8. PMID: 10882760
- An ATP-binding cassette gene (ABCG5) from the ABCG (White) gene subfamily maps to human chromosome 2p21 in the region of the Sitosterolemia locus. | Shulenin, S., et al. 2001. Cytogenet Cell Genet. 92: 204-8. PMID: 11435688
- Mutation of ALMS1, a large gene with a tandem repeat encoding 47 amino acids, causes Alström syndrome. | Hearn, T., et al. 2002. Nat Genet. 31: 79-83. PMID: 11941370
- An unusual peptide deformylase features in the human mitochondrial N-terminal methionine excision pathway. | Serero, A., et al. 2003. J Biol Chem. 278: 52953-63. PMID: 14532271
- Mutations in ABCA12 underlie the severe congenital skin disease harlequin ichthyosis. | Kelsell, DP., et al. 2005. Am J Hum Genet. 76: 794-803. PMID: 15756637
- MAP1D, a novel methionine aminopeptidase family member is overexpressed in colon cancer. | Leszczyniecka, M., et al. 2006. Oncogene. 25: 3471-8. PMID: 16568094
- Kinetic and mutational studies of the number of interacting divalent cations required by bacterial and human methionine aminopeptidases. | Hu, XV., et al. 2007. Biochemistry. 46: 12833-43. PMID: 17929833
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAP1D Antibody (B-8) | sc-515155 | 200 µg/ml | $322.00 | |||
MAP1D Antibody (B-8): m-IgGκ BP-HRP Bundle | sc-524958 | 200 µg Ab, 40 µg BP | $361.00 | |||
MAP1D Antibody (B-8): m-IgG1 BP-HRP Bundle | sc-533643 | 200 µg Ab; 20 µg BP | $361.00 | |||
MAP1D Antibody (B-8) AC | sc-515155 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MAP1D Antibody (B-8) HRP | sc-515155 HRP | 200 µg/ml | $322.00 | |||
MAP1D Antibody (B-8) FITC | sc-515155 FITC | 200 µg/ml | $336.00 | |||
MAP1D Antibody (B-8) PE | sc-515155 PE | 200 µg/ml | $349.00 | |||
MAP1D Antibody (B-8) Alexa Fluor® 488 | sc-515155 AF488 | 200 µg/ml | $364.00 | |||
MAP1D Antibody (B-8) Alexa Fluor® 546 | sc-515155 AF546 | 200 µg/ml | $364.00 | |||
MAP1D Antibody (B-8) Alexa Fluor® 594 | sc-515155 AF594 | 200 µg/ml | $364.00 | |||
MAP1D Antibody (B-8) Alexa Fluor® 647 | sc-515155 AF647 | 200 µg/ml | $364.00 | |||
MAP1D Antibody (B-8) Alexa Fluor® 680 | sc-515155 AF680 | 200 µg/ml | $364.00 | |||
MAP1D Antibody (B-8) Alexa Fluor® 790 | sc-515155 AF790 | 200 µg/ml | $364.00 |