MAP-4 Antibody (G-10): sc-390286

MAP-4 Antibody (G-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects MAP-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAP-4 (G-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAP-4 plays a crucial role in the assembly and stability of microtubules, which are essential components of the cytoskeletal network. Microtubules are vital for various cellular processes, including maintaining cell shape, enabling intracellular transport, and facilitating cell division. MAP-4, a non-neuronal microtubule-associated protein, contains three 18-amino acid repeats that are homologous to those found in other microtubule-associated proteins. This structural feature allows MAP-4 to interact with tubulin and other MAPs, thereby promoting microtubule assembly and stability. MAP-4 is predominantly expressed in tissues such as kidney, lung, liver, testis, and spleen, where MAP-4 contributes to the organization and dynamics of the microtubule network during critical cellular events, including interphase and mitosis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MAP-4 Antibody (G-10) References:

1. Analysis of microtubule organization and dynamics in living cells using green fluorescent protein-microtubule-associated protein 4 chimeras.  |  Olson, KR. and Olmsted, JB. 1999. Methods Enzymol. 302: 103-20. PMID: 12876766
2. Interphase and monoastral-mitotic phenotypes of overexpressed MAP4 are modulated by free tubulin concentrations.  |  Holmfeldt, P., et al. 2003. J Cell Sci. 116: 3701-11. PMID: 12890753
3. In vitro study of cell-promoting multiple-armed peptides.  |  Li, C., et al. 2004. J Biomed Mater Res A. 71: 134-42. PMID: 15368263
4. Modulation of gene expression in human central nervous system tumors under methionine deprivation-induced stress.  |  Kokkinakis, DM., et al. 2004. Cancer Res. 64: 7513-25. PMID: 15492278
5. Inhibition of beta-adrenergic receptor trafficking in adult cardiocytes by MAP4 decoration of microtubules.  |  Cheng, G., et al. 2005. Am J Physiol Heart Circ Physiol. 288: H1193-202. PMID: 15528234
6. Autogenic modulation of mechanoreceptor excitability by glutamate release from synaptic-like vesicles: evidence from the rat muscle spindle primary sensory ending.  |  Bewick, GS., et al. 2005. J Physiol. 562: 381-94. PMID: 15528245
7. Genetic modification of the heart: chaperones and the cytoskeleton.  |  Kumarapeli, AR. and Wang, X. 2004. J Mol Cell Cardiol. 37: 1097-109. PMID: 15572040
8. The MAP2/Tau family of microtubule-associated proteins.  |  Dehmelt, L. and Halpain, S. 2005. Genome Biol. 6: 204. PMID: 15642108
9. Characterization of long-term potentiation of primary afferent transmission at trigeminal synapses of juvenile rats: essential role of subtype 5 metabotropic glutamate receptors.  |  Liang, YC., et al. 2005. Pain. 114: 417-428. PMID: 15777867
10. A model for microtubule-associated protein 4 structure. Domains defined by comparisons of human, mouse, and bovine sequences.  |  West, RR., et al. 1991. J Biol Chem. 266: 21886-96. PMID: 1718985
11. Non-neuronal 210 x 10(3) Mr microtubule-associated protein (MAP4) contains a domain homologous to the microtubule-binding domains of neuronal MAP2 and tau.  |  Chapin, SJ. and Bulinski, JC. 1991. J Cell Sci. 98 (Pt 1): 27-36. PMID: 1905296
12. A muscle-specific variant of microtubule-associated protein 4 (MAP4) is required in myogenesis.  |  Mangan, ME. and Olmsted, JB. 1996. Development. 122: 771-81. PMID: 8631255

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MAP-4 Antibody (G-10) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-MAP-4 Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MAP-4 Antibody (G-10) Specifications

Alternate Product Name: MAP-4

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: MAP-4

Epitope Species: mouse

Product Reactivity/Detection: MAP-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for MAP-4 Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin