MAP-4 Antibody (E-1): sc-365492

MAP-4 Antibody (E-1) is a mouse monoclonal IgM antibody that detects MAP-4 in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAP-4 is a crucial microtubule-associated protein that plays a significant role in the assembly and stability of microtubules, which are essential components of the cytoskeletal network. MAP-4 is primarily expressed in vital organs such as the kidney, lung, liver, testis, and spleen, where MAP-4 contributes to the organization and function of microtubules during various cellular processes, including interphase and mitosis. Notably, MAP-4 contains three 18-amino-acid repeats that are homologous to those found in other microtubule-associated proteins, underscoring MAP-4′s structural importance and evolutionary conservation. MAP-4′s ability to enhance microtubule stability and influence their spatial distribution is critical for proper cellular function, as MAP-4 facilitates the translocation of cellular components and ensures accurate mitotic movements. By utilizing anti-MAP-4 antibody (E-1), researchers can gain valuable insights into the dynamics of microtubule organization and its implications in cellular processes and disease states.

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MAP-4 Antibody (E-1) References:

1. Microtubule-binding property of microtubule-associated protein 2 differs from that of microtubule-associated protein 4 and tau.  |  Tokuraku, K., et al. 1999. Eur J Biochem. 264: 996-1001. PMID: 10491150
2. The number of repeat sequences in microtubule-associated protein 4 affects the microtubule surface properties.  |  Tokuraku, K., et al. 2003. J Biol Chem. 278: 29609-18. PMID: 12773533
3. Mitogen-activated-protein-kinase-catalyzed phosphorylation of microtubule-associated proteins, microtubule-associated protein 2 and microtubule-associated protein 4, induces an alteration in their function.  |  Hoshi, M., et al. 1992. Eur J Biochem. 203: 43-52. PMID: 1309698
4. Up-regulation of microtubule-associated protein 4 and drebrin A mRNA expression by antidepressants in rat hippocampus following chronic stress.  |  Yang, HJ., et al. 2003. Neurosci Lett. 351: 206-8. PMID: 14623141
5. Mammalian septins regulate microtubule stability through interaction with the microtubule-binding protein MAP4.  |  Kremer, BE., et al. 2005. Mol Biol Cell. 16: 4648-59. PMID: 16093351
6. Site-specific phosphorylation by protein kinase C inhibits assembly-promoting activity of microtubule-associated protein 4.  |  Mori, A., et al. 1991. Biochemistry. 30: 9341-6. PMID: 1892837
7. Non-neuronal 210 x 10(3) Mr microtubule-associated protein (MAP4) contains a domain homologous to the microtubule-binding domains of neuronal MAP2 and tau.  |  Chapin, SJ. and Bulinski, JC. 1991. J Cell Sci. 98 (Pt 1): 27-36. PMID: 1905296
8. Knockdown of MAP4 and DNAL1 produces a post-fusion and pre-nuclear translocation impairment in HIV-1 replication.  |  Gallo, DE. and Hope, TJ. 2012. Virology. 422: 13-21. PMID: 22018492
9. FBXW7 loss of function promotes esophageal squamous cell carcinoma progression via elevating MAP4 and ERK phosphorylation.  |  Pan, Y., et al. 2023. J Exp Clin Cancer Res. 42: 75. PMID: 36991467
10. The gene for microtubule-associated protein 4 (Mtap4) maps to the distal region of mouse chromosome 9.  |  Mangan, ME. and Olmsted, JB. 1996. Mamm Genome. 7: 918-9. PMID: 8995766

The preferred MAP-4 antibody is MAP-4 Antibody (G-10): sc-390286.