Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAO-A CRISPR/Cas9 KO Plasmid (h): sc-401030
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAO-A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MAO-A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAO-A Antibody (G-10): sc-271123
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAO-A CRISPR/Cas9 KO Plasmid (h) | sc-401030 | 20 µg | $397.00 |
Overview
Human MAOA encodes mitochondrial outer membrane monoamine oxidase A (MAO-A), a flavin-dependent enzyme that catalyzes oxidative deamination of biogenic amines including serotonin, norepinephrine, and dopamine. Through regulation of monoamine catabolism, MAO-A influences neurotransmitter homeostasis, mitochondrial redox balance, and downstream signaling networks linked to cellular stress responses. MAO-A activity contributes to pathways affecting reactive oxygen species production and aldehyde metabolism, connecting monoamine turnover to oxidative stress and metabolic adaptation. Altered MAOA expression or function has been associated with neuropsychiatric phenotypes and disorders involving dysregulated monoaminergic signaling, making it a relevant target for mechanistic studies in neural and peripheral cell systems.
MAO-A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MAOA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MAOA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MAOA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MAO-A protein expression.
This CRISPR knockout system enables efficient generation of MAOA-deficient cell models for investigation of MAO-A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MAOA exon(s) critical for MAO-A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MAOA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MAO-A CRISPR/Cas9 KO Plasmid (h) and MAO-A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MAOA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MAO-A HDR Plasmid (h) and MAO-A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MAOA homology arms to support homology-directed repair at defined MAOA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.