Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAN2C1 Double Nickase Plasmid (h): sc-404066-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAN2C1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MAN2C1 Double Nickase Plasmid (h) and MAN2C1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAN2C1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAN2C1 Antibody (C-4): sc-377132
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAN2C1 Double Nickase Plasmid (h) | sc-404066-NIC | 20 µg | $410.00 | |||
MAN2C1 Double Nickase Plasmid (h2) | sc-404066-NIC-2 | 20 µg | $410.00 |
MAN2C1 encodes cytosolic alpha-mannosidase 2C1, an exo-glycosidase that trims free oligosaccharides generated during glycoprotein quality control and the clearance of misfolded proteins. By processing high-mannose N-glycan fragments outside the lysosome, MAN2C1 contributes to carbohydrate catabolism and helps regulate the cellular burden of deglycosylated glycans arising from ER-associated degradation. Altered MAN2C1 activity can influence proteostasis and glycan-dependent signaling networks, linking this enzyme to pathways that modulate cellular stress responses and metabolic homeostasis. Dysregulation of glycosylation and free oligosaccharide turnover is frequently observed in human disease contexts, supporting the use of MAN2C1 perturbation in mechanistic studies of glycoprotein processing.
MAN2C1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAN2C1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAN2C1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAN2C1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAN2C1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.