MAN2A1 Antibody (F-10): sc-376909

MAN2A1 Antibody (F-10) is a mouse monoclonal IgG1 antibody that detects MAN2A1 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MAN2A1 (F-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAN2A1, also known as mannosidase alpha class 2A member 1, is a single-pass type II membrane protein primarily located in the cisternae of the Golgi apparatus, where MAN2A1 plays a crucial role in glycoprotein modification. Specifically, MAN2A1 is involved in N-glycan processing, catalyzing the hydrolysis of terminal alpha-D-mannose residues in the oligosaccharide Man5(GlcNAc)3, which is a vital step in glycoprotein maturation. This enzymatic activity is essential for proper protein folding and function, as well as for overall glycoprotein quality control within cells. MAN2A1′s role in glycan processing highlights potential implications in various biological processes and diseases, making MAN2A1 a valuable target for research and therapeutic applications.

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MAN2A1 Antibody (F-10) References:

1. Genetic remodeling of protein glycosylation in vivo induces autoimmune disease.  |  Chui, D., et al. 2001. Proc Natl Acad Sci U S A. 98: 1142-7. PMID: 11158608
2. Glycosylation inhibitors and neuraminidase enhance human immunodeficiency virus type 1 binding and neutralization by mannose-binding lectin.  |  Hart, ML., et al. 2003. J Gen Virol. 84: 353-360. PMID: 12560567
3. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.  |  Liu, T., et al. 2005. J Proteome Res. 4: 2070-80. PMID: 16335952
4. Essential and mutually compensatory roles of {alpha}-mannosidase II and {alpha}-mannosidase IIx in N-glycan processing in vivo in mice.  |  Akama, TO., et al. 2006. Proc Natl Acad Sci U S A. 103: 8983-8. PMID: 16754854
5. Disruption of alpha-mannosidase processing induces non-canonical hybrid-type glycosylation.  |  Crispin, M., et al. 2007. FEBS Lett. 581: 1963-8. PMID: 17466984
6. Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans.  |  Moremen, KW. and Robbins, PW. 1991. J Cell Biol. 115: 1521-34. PMID: 1757461
7. Novel fusion transcripts associate with progressive prostate cancer.  |  Yu, YP., et al. 2014. Am J Pathol. 184: 2840-9. PMID: 25238935
8. Intestinal Epithelial Cell-specific Deletion of α-Mannosidase II Ameliorates Experimental Colitis.  |  Suzuki, K., et al. 2018. Cell Struct Funct. 43: 25-39. PMID: 29343654
9. Detection of fusion transcripts in the serum samples of patients with hepatocellular carcinoma.  |  Yu, YP., et al. 2019. Oncotarget. 10: 3352-3360. PMID: 31164957
10. Cell engineering for the production of hybrid-type N-glycans in HEK293 cells.  |  Leng, JX., et al. 2021. J Biochem. 170: 139-151. PMID: 33878161
11. Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase IIx isozyme.  |  Misago, M., et al. 1995. Proc Natl Acad Sci U S A. 92: 11766-70. PMID: 8524845
12. Alpha-mannosidase-II deficiency results in dyserythropoiesis and unveils an alternate pathway in oligosaccharide biosynthesis.  |  Chui, D., et al. 1997. Cell. 90: 157-67. PMID: 9230311

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MAN2A1 Antibody (F-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-MAN2A1 Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MAN2A1 Antibody (F-10) Specifications

Alternate Product Name: MAN2A1

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 583-806

Epitope Target: MAN2A1

Epitope Species: mouse

Product Reactivity/Detection: MAN2A1

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for MAN2A1 Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin