MAML3 Antibody (797C2a): sc-81104

MAML3 Antibody (797C2a) is a mouse monoclonal IgG1 antibody that detects MAML3 protein of human origin by western blotting (WB) and immunoprecipitation (IP). Anti-MAML3 antibody (797C2a) is available as a non-conjugated format. MAML3, or Mastermind-like protein 3, is a crucial nuclear speckle protein that functions as a transcriptional coactivator for Notch receptors, playing a significant role in the Notch signaling pathway, which is essential for determining cell fate by regulating how precursor cells respond to developmental signals. MAML3′s structure consists of an N-terminal domain that is vital for binding to the ankyrin repeats of Notch proteins (Notch 1, Notch 2, Notch 3, and Notch 4) and a C-terminal domain that is critical for transcriptional activation. This structural arrangement allows MAML3 to form a DNA-binding complex with Notch proteins and RBP-Jκ, which subsequently induces HES1 gene expression. The precise interaction between these domains is crucial for MAML3′s function in cell signaling and transcriptional regulation. Notably, MAML3 up-regulation has been implicated in oncogenesis, highlighting MAML3′s potential role in cancer development.

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MAML3 Antibody (797C2a) References:

1. Identification of a family of mastermind-like transcriptional coactivators for mammalian notch receptors.  |  Wu, L., et al. 2002. Mol Cell Biol. 22: 7688-700. PMID: 12370315
2. Identification of new human mastermind proteins defines a family that consists of positive regulators for notch signaling.  |  Lin, SE., et al. 2002. J Biol Chem. 277: 50612-20. PMID: 12386158
3. Modulation of Notch signaling by mastermind-like (MAML) transcriptional co-activators and their involvement in tumorigenesis.  |  Wu, L. and Griffin, JD. 2004. Semin Cancer Biol. 14: 348-56. PMID: 15288260
4. WNT antagonist, DKK2, is a Notch signaling target in intestinal stem cells: augmentation of a negative regulation system for canonical WNT signaling pathway by the Notch-DKK2 signaling loop in primates.  |  Katoh, M. and Katoh, M. 2007. Int J Mol Med. 19: 197-201. PMID: 17143565
5. Identification of oscillatory genes in somitogenesis from functional genomic analysis of a human mesenchymal stem cell model.  |  William, DA., et al. 2007. Dev Biol. 305: 172-86. PMID: 17362910
6. The transcriptional coactivator Maml1 is required for Notch2-mediated marginal zone B-cell development.  |  Wu, L., et al. 2007. Blood. 110: 3618-23. PMID: 17699740