Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MALT1 Double Nickase Plasmid (m): sc-433761-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MALT1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MALT1 Double Nickase Plasmid (m) and MALT1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Malt1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MALT1 Antibody (D-1): sc-515389
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MALT1 Double Nickase Plasmid (m) | sc-433761-NIC | 20 µg | $410.00 |
Malt1 encodes MALT1 (paracaspase), a core scaffold and protease within the CARD11–BCL10–MALT1 (CBM) signalosome that links antigen receptor engagement to canonical NF-κB and MAPK pathway activation. In lymphocytes, MALT1 integrates upstream PKC signaling to promote IKK activation and transcriptional programs controlling survival, proliferation, and cytokine production. Its protease activity modulates signaling amplitude by cleaving negative regulators and shaping inflammatory outputs. Dysregulated MALT1 signaling is widely studied in immune-mediated inflammation and lymphoid malignancy biology, making it a key node for mechanistic studies of immune signaling networks.
MALT1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Malt1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Malt1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Malt1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Malt1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.