MAL Double Nickase Plasmid (h2): sc-403024-NIC-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAL Double Nickase Plasmid (h2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MAL Double Nickase Plasmid (h2) and MAL Double Nickase Plasmid (h22) encode distinct paired gRNA designs targeting MAL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAL Antibody (E-1): sc-390687
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAL Double Nickase Plasmid (h2) | sc-403024-NIC-2 | 20 µg | $410.00 |
Human MAL encodes the myelin and lymphocyte protein, a highly hydrophobic tetraspan membrane component enriched in glycolipid- and cholesterol-rich membrane microdomains that helps organize apical sorting and vesicular trafficking in polarized epithelia and contributes to proteolipid organization in myelinating cells. MAL participates in membrane raft–dependent transport processes and influences endocytic recycling and signal compartmentalization, linking it to epithelial polarity maintenance and specialized membrane domain formation. Altered MAL expression or epigenetic silencing has been reported across multiple cancers and in contexts of demyelination and immune cell function, making it a useful marker for studying lineage differentiation, membrane trafficking defects, and tumor biology. Gene editing of MAL supports mechanistic investigation of raft-associated transport pathways, cell polarity regulation, and context-specific effects on proliferation, migration, and myelin-related membrane dynamics in human model systems.
MAL Double Nickase Plasmid (h2) consists of a matched pair of plasmids engineered for high-specificity editing of the MAL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.