MAGP-1 Antibody (G-7): sc-166075

MAGP-1 Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects MAGP-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MAGP-1 (G-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAGP-1 plays a crucial role in forming and maintaining elastic fibers, which are essential for loose connective tissue resilience, complementing tensile strength provided by collagen fibers. Elastic fibers primarily consist of elastin and a microfibril network, including glycoproteins such as fibrillin-1, fibrillin-2, and microfibril-associated glycoproteins like MAGP-1 and MAGP-2. During elastogenesis, MAGP-1 and MAGP-2 facilitate fibrillin binding to tropoelastin within extracellular matrix of various elastic and non-elastic tissues. MAGP-1 functions as an O-glycosylated protein secreted into extracellular space and matrix, forming a ternary complex with Biglycan and elastin. MAGP-1 can create intermolecular disulfide bonds with other MAGP-1 molecules or microfibril components, potentially leading to transglutaminase cross-linking. Studies demonstrate that both underexpression and overexpression of Zebrafish homolog, Magp-1, significantly impact vascular development, highlighting MAGP-1′s critical role in tissue integrity and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MAGP-1 Antibody (G-7) References:

1. Identification of a matrix-binding domain in MAGP1 and MAGP2 and intracellular localization of alternative splice forms.  |  Segade, F., et al. 2002. J Biol Chem. 277: 11050-7. PMID: 11796718
2. Microfibril-associated glycoprotein-2 interacts with fibrillin-1 and fibrillin-2 suggesting a role for MAGP-2 in elastic fiber assembly.  |  Penner, AS., et al. 2002. J Biol Chem. 277: 35044-9. PMID: 12122015
3. Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro.  |  Tsuruga, E., et al. 2005. Int J Biochem Cell Biol. 37: 120-9. PMID: 15381155
4. Functional analysis of zebrafish microfibril-associated glycoprotein-1 (Magp1) in vivo reveals roles for microfibrils in vascular development and function.  |  Chen, E., et al. 2006. Blood. 107: 4364-74. PMID: 16469878
5. The protein components of the 12-nanometer microfibrils of elastic and nonelastic tissues.  |  Gibson, MA., et al. 1989. J Biol Chem. 264: 4590-8. PMID: 2647740
6. The tissue distribution of microfibrils reacting with a monospecific antibody to MAGP, the major glycoprotein antigen of elastin-associated microfibrils.  |  Kumaratilake, JS., et al. 1989. Eur J Cell Biol. 50: 117-27. PMID: 2693088
7. Isolation and characterization of a new 36-kDa microfibril-associated glycoprotein from porcine aorta.  |  Kobayashi, R., et al. 1989. J Biol Chem. 264: 17437-44. PMID: 2793866
8. Microfibril-associated glycoproteins MAGP-1 and MAGP-2 in disease.  |  Craft, CS., et al. 2018. Matrix Biol. 71-72: 100-111. PMID: 29524629
9. MAGP-1 and fibronectin control EGFL7 functions by driving its deposition into distinct endothelial extracellular matrix locations.  |  Villain, G., et al. 2018. FEBS J. 285: 4394-4412. PMID: 30338930
10. Identification of the growth factor-binding sequence in the extracellular matrix protein MAGP-1.  |  Broekelmann, TJ., et al. 2020. J Biol Chem. 295: 2687-2697. PMID: 31988245
11. The immunohistochemical localisation of microfibril-associated glycoprotein (MAGP) in elastic and non-elastic tissues.  |  Gibson, MA. and Cleary, EG. 1987. Immunol Cell Biol. 65 (Pt 4): 345-56. PMID: 3315986
12. Decreased Levels of Microfibril-Associated Glycoprotein (MAGP)-1 in Patients with Colon Cancer and Obesity Are Associated with Changes in Extracellular Matrix Remodelling.  |  Gómez de Segura, I., et al. 2021. Int J Mol Sci. 22: PMID: 34445187

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MAGP-1 Antibody (G-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-MAGP-1 Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MAGP-1 Antibody (G-7) Specifications

Alternate Product Name: MAGP-1

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 32-61

Epitope Target: MAGP-1

Epitope Species: human

Product Reactivity/Detection: MAGP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for MAGP-1 Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin