MafF/G/K Antibody (D-12): sc-166548

MafF/G/K Antibody (D-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects MafF/G/K protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MafF/G/K (D-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MafF/G/K protein plays a crucial role in gene expression regulation as a member of the small Maf family of basic region/leucine zipper (bZIP) transcription factors, which form heterodimers with larger tissue-restricted subunits like p45, a component of NF-E2. This interaction is vital for transcriptional regulation of erythroid-specific genes, essential for proper red blood cell development and function. MafF expression is particularly prominent in the lung, where MafF compensates for loss of function mutations in MafG and MafK, ensuring maintenance of critical physiological processes. Small Maf proteins, including MafF, MafG, and MafK, are implicated in various developmental and physiological roles, highlighting their importance in cellular responses to stress and differentiation. Using anti-MafF/G/K antibody (D-12) in research can provide valuable insights into complex regulatory mechanisms governing gene expression and functional dynamics of the Maf family in different biological contexts.

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MafF/G/K Antibody (D-12) References:

1. Regulation of mouse lens fiber cell development and differentiation by the Maf gene.  |  Ring, BZ., et al. 2000. Development. 127: 307-17. PMID: 10603348
2. Differential induction of mafF, mafG and mafK expression by electrophile-response-element activators.  |  Moran, JA., et al. 2002. Biochem J. 361: 371-7. PMID: 11772409
3. The novel human gene MIP functions as a co-activator of hMafF.  |  Ye, X., et al. 2006. Arch Biochem Biophys. 449: 87-93. PMID: 16549056
4. MafG sumoylation is required for active transcriptional repression.  |  Motohashi, H., et al. 2006. Mol Cell Biol. 26: 4652-63. PMID: 16738329
5. MafK overexpression in pancreatic beta-cells caused impairment of glucose-stimulated insulin secretion.  |  Shimohata, H., et al. 2006. Biochem Biophys Res Commun. 346: 671-80. PMID: 16780794
6. Small Maf proteins (MafF, MafG, MafK): History, structure and function.  |  Katsuoka, F. and Yamamoto, M. 2016. Gene. 586: 197-205. PMID: 27058431
7. Conditional expression of the ubiquitous transcription factor MafK induces erythroleukemia cell differentiation.  |  Igarashi, K., et al. 1995. Proc Natl Acad Sci U S A. 92: 7445-9. PMID: 7638211
8. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.  |  Kataoka, K., et al. 1995. Mol Cell Biol. 15: 2180-90. PMID: 7891713
9. A conserved region adjacent to the basic domain is required for recognition of an extended DNA binding site by Maf/Nrl family proteins.  |  Kerppola, TK. and Curran, T. 1994. Oncogene. 9: 3149-58. PMID: 7936637
10. Small Maf proteins interact with the human transcription factor TCF11/Nrf1/LCR-F1.  |  Johnsen, O., et al. 1996. Nucleic Acids Res. 24: 4289-97. PMID: 8932385
11. c-Maf interacts with c-Myb to regulate transcription of an early myeloid gene during differentiation.  |  Hedge, SP., et al. 1998. Mol Cell Biol. 18: 2729-37. PMID: 9566892
12. Rat maf-related factors: the specificities of DNA binding and heterodimer formation.  |  Matsushima-Hibiya, Y., et al. 1998. Biochem Biophys Res Commun. 245: 412-8. PMID: 9571165

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MafF/G/K Antibody (D-12) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-MafF/G/K Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MafF/G/K Antibody (D-12) Specifications

Alternate Product Name: MafF/G/K

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 63-126

Epitope Target: MafG

Epitope Species: human

Product Reactivity/Detection: MafF, MafG and NF-E2 p18 (also designated MafK)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for MafF/G/K Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent