MafB (h2): 293T Lysate: sc-114754
- human MafB transfected 293T lysate
- 100 µg protein in 200 µl SDS-PAGE buffer; suitable as Western Blotting control for MafB antibodies
- Suitable control antibody: MafB Antibody (F-11): sc-74521 is recommended as control antibody for monitoring of MafB expression knockdown by Western blotting or immunofluorescence
- should be stored at -20° C and repeated freezing and thawing should be minimized
- sample vial should be boiled once prior to use
- non-transfected control 293T lysate (sc-117752)
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MafB (h2): 293T Lysate, derived from the HEK 293T cell line, is extensively utilized in the study of the MafB transcription factor, focusing on its role in regulating gene expression linked to cellular differentiation and development. The 293T cell line, modified to enhance plasmid replication and protein expression due to the SV40 large T antigen, provides a robust platform for expressing transcription factors like MafB. Researchers use this lysate to investigate MafB's specific binding to MARE (Maf recognition element) sequences within the promoter regions of target genes, which influences the transcription of genes involved in the development of various cell lineages, especially in the hematopoietic and myeloid systems. Techniques such as chromatin immunoprecipitation (ChIP), luciferase reporter assays, and electrophoretic mobility shift assays (EMSAs) are commonly used to study these interactions. This research provides insight into the fundamental mechanisms by which MafB contributes to gene regulatory networks, enhancing our understanding of transcriptional control in cellular development processes.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MafB (h2): 293T Lysate | sc-114754 | 100 µg/200 µl | $232.00 |