MafA Antibody (F-6): sc-390491

MafA Antibody (F-6) is a mouse monoclonal IgM antibody that detects MafA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MafA (F-6) antibody is available as the non-conjugated form. MafA protein, a member of the bZIP transcription factor family, plays a pivotal role in the differentiation and regulation of pancreatic β-cells, where MafA acts as a transcriptional activator essential for insulin gene expression. Specifically, MafA protein binds to the insulin enhancer element RIPE3b, facilitating the glucose-responsive expression of insulin, which is crucial for maintaining glucose homeostasis in the body. MafA protein′s importance extends beyond insulin production; MafA is also involved in the development of pancreatic β-cells and their functional maturation. Notably, MafA protein size can vary in mammalian cell lines due to post-translational modifications, which can influence activity and stability, making the study of these modifications vital for understanding regulatory mechanisms.

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MafA Antibody (F-6) References:

1. Identification of beta-cell-specific insulin gene transcription factor RIPE3b1 as mammalian MafA.  |  Olbrot, M., et al. 2002. Proc Natl Acad Sci U S A. 99: 6737-42. PMID: 12011435
2. MafA is a glucose-regulated and pancreatic beta-cell-specific transcriptional activator for the insulin gene.  |  Kataoka, K., et al. 2002. J Biol Chem. 277: 49903-10. PMID: 12368292
3. The islet beta cell-enriched RIPE3b1/Maf transcription factor regulates pdx-1 expression.  |  Samaras, SE., et al. 2003. J Biol Chem. 278: 12263-70. PMID: 12551916
4. MafA has strong cell transforming ability but is a weak transactivator.  |  Nishizawa, M., et al. 2003. Oncogene. 22: 7882-90. PMID: 12970735
5. The MafA transcription factor appears to be responsible for tissue-specific expression of insulin.  |  Matsuoka, TA., et al. 2004. Proc Natl Acad Sci U S A. 101: 2930-3. PMID: 14973194
6. Regulation of β-cell-specific and glucose-dependent MafA expression.  |  Vanderford, NL. 2011. Islets. 3: 35-7. PMID: 21278484
7. MafA and MafB activity in pancreatic β cells.  |  Hang, Y. and Stein, R. 2011. Trends Endocrinol Metab. 22: 364-73. PMID: 21719305
8. Proper activation of MafA is required for optimal differentiation and maturation of pancreatic β-cells.  |  El Khattabi, I. and Sharma, A. 2015. Best Pract Res Clin Endocrinol Metab. 29: 821-31. PMID: 26696512
9. MAFA and MAFB regulate exocytosis-related genes in human β-cells.  |  Cataldo, LR., et al. 2022. Acta Physiol (Oxf). 234: e13761. PMID: 34978761
10. Role of the Transcription Factor MAFA in the Maintenance of Pancreatic β-Cells.  |  Nishimura, W., et al. 2022. Int J Mol Sci. 23: PMID: 35562869
11. The ubiquitin ligase HERC4 suppresses MafA transcriptional activity triggered by GSK3β in myeloma by atypical K63-linked polyubiquitination.  |  Zhang, Z., et al. 2023. J Biol Chem. 299: 104675. PMID: 37028761
12. A conserved region adjacent to the basic domain is required for recognition of an extended DNA binding site by Maf/Nrl family proteins.  |  Kerppola, TK. and Curran, T. 1994. Oncogene. 9: 3149-58. PMID: 7936637

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MafA Antibody (F-6) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-MafA Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MafA Antibody (F-6) Specifications

Alternate Product Name: MAF BZIP Transcription Factor A

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 330-341

Epitope Target: MafA

Epitope Species: human

Product Reactivity/Detection: MafA

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for MafA Antibody (F-6): P: Blocking Peptide, X: TransCruz Reagent