Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAD2B Antibody (14): sc-135977. Western blot analysis of MAD2B expression in non-transfected 293: sc-110760 (A), human MAD2B transfected 293: sc-113252 (B), K-562 (C), HeLa (D) and Neuro-2A (E) whole cell lysates and mouse embryo tissue extract (F).
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAD2B Double Nickase Plasmid (m): sc-428124-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAD2B Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MAD2B Double Nickase Plasmid (m) and MAD2B Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Mad2l2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAD2B Antibody (14): sc-135977
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAD2B Double Nickase Plasmid (m) | sc-428124-NIC | 20 µg | $410.00 | |||
MAD2B Double Nickase Plasmid (m2) | sc-428124-NIC-2 | 20 µg | $410.00 |