MAD1 Antibody (G-12): sc-166322
- MAD1 Antibody (G-12) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 491-718 mapping at the C-terminus of MAD1 of human origin
- recommended for detection of MAD1 of human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for MAD1 Antibody (G-12) for WB applications. These reagents are now offered in bundles with MAD1 Antibody (G-12) (see ordering information below).
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MAD1 Antibody (G-12) is a mouse monoclonal IgG2b antibody that detects MAD1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAD1 plays a critical role in the regulation of the cell cycle, particularly at the mitotic spindle assembly checkpoint, where MAD1 ensures that all chromosomes are properly aligned before the cell proceeds to anaphase. This function is vital for maintaining genomic stability, as errors in chromosome segregation can lead to aneuploidy and contribute to cancer development. MAD1 is primarily localized in the nucleus, but MAD1′s dynamic movement during mitosis to the centrosome, spindle midzone, and midbody highlights MAD1′s importance in coordinating the mitotic process. Additionally, MAD1 interacts with MAD2 to form a tetrameric core complex, which is essential for MAD1′s checkpoint function. MAD1′s activity is regulated by phosphorylation, particularly in response to spindle damage, underscoring MAD1′s role as a key player in the cellular response to mitotic stress. Defects in the MAD1 gene have been implicated in various cancer types, making anti-MAD1 antibody (G-12) a valuable tool for researchers studying cell cycle regulation and cancer biology.
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MAD1 Antibody (G-12) References:
- The Mad1-Mad2 balancing act--a damaged spindle checkpoint in chromosome instability and cancer. | Schuyler, SC., et al. 2012. J Cell Sci. 125: 4197-206. PMID: 23093575
- Dual personality of Mad1: regulation of nuclear import by a spindle assembly checkpoint protein. | Cairo, LV., et al. 2013. Nucleus. 4: 367-73. PMID: 24076561
- Mitotic checkpoint proteins Mad1 and Mad2 - structural and functional relationship with implication in genetic diseases. | Avram, S., et al. 2014. Curr Comput Aided Drug Des. 10: 168-81. PMID: 24724894
- The spindle assembly checkpoint promotes chromosome bi-orientation: A novel Mad1 role in chromosome alignment. | Akera, T. and Watanabe, Y. 2016. Cell Cycle. 15: 493-7. PMID: 26752263
- Mad1 destabilizes p53 by preventing PML from sequestering MDM2. | Wan, J., et al. 2019. Nat Commun. 10: 1540. PMID: 30948704
- Cyclin B1 scaffolds MAD1 at the kinetochore corona to activate the mitotic checkpoint. | Allan, LA., et al. 2020. EMBO J. 39: e103180. PMID: 32202322
- Molecular mechanism of Mad1 kinetochore targeting by phosphorylated Bub1. | Fischer, ES., et al. 2021. EMBO Rep. 22: e52242. PMID: 34013668
- Juxtaposition of Bub1 and Cdc20 on phosphorylated Mad1 during catalytic mitotic checkpoint complex assembly. | Fischer, ES., et al. 2022. Nat Commun. 13: 6381. PMID: 36289199
- The structural flexibility of MAD1 facilitates the assembly of the Mitotic Checkpoint Complex. | Chen, C., et al. 2023. Nat Commun. 14: 1529. PMID: 36934097
- MAD1 upregulation sensitizes to inflammation-mediated tumor formation. | Copeland, SE., et al. 2024. PLoS Genet. 20: e1011437. PMID: 39374311
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAD1 Antibody (G-12) | sc-166322 | 200 µg/ml | $322.00 | |||
MAD1 Antibody (G-12): m-IgG Fc BP-HRP Bundle | sc-540076 | 200 µg Ab; 10 µg BP | $361.00 | |||
MAD1 Antibody (G-12): m-IgG2b BP-HRP Bundle | sc-549771 | 200 µg Ab; 10 µg BP | $361.00 |