Mad 1 Antibody (F-1): sc-8012

Mad 1 Antibody (F-1) is a mouse monoclonal IgG1 antibody that detects Mad 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Mad 1 (F-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Mad 1 protein plays a crucial role in the regulation of gene expression as a transcriptional repressor, particularly in cell differentiation and proliferation. Mad 1 protein functions by forming heterodimeric complexes with Max, which antagonize Myc-Max heterodimers′ activity, thereby inhibiting genes that promote cell growth and division. Mad 1 protein′s regulatory mechanism is vital for maintaining cellular homeostasis and preventing uncontrolled cell proliferation, which can lead to oncogenesis. Mad 1 protein′s stability, in contrast to highly regulated Myc protein, allows effective modulation of gene expression in response to various cellular signals. Understanding Mad 1 protein dynamics and interactions with other proteins is essential for elucidating complex networks that govern cellular behavior and developing potential therapeutic strategies targeting these pathways.

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Mad 1 Antibody (F-1) References:

1. Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants.  |  Mukherjee, B., et al. 1992. Genes Dev. 6: 1480-92. PMID: 1644290
2. Association of Myn, the murine homolog of max, with c-Myc stimulates methylation-sensitive DNA binding and ras cotransformation.  |  Prendergast, GC., et al. 1991. Cell. 65: 395-407. PMID: 1840505
3. Intracellular leucine zipper interactions suggest c-Myc hetero-oligomerization.  |  Dang, CV., et al. 1991. Mol Cell Biol. 11: 954-62. PMID: 1990293
4. Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc.  |  Blackwood, EM. and Eisenman, RN. 1991. Science. 251: 1211-7. PMID: 2006410
5. Transcriptional regulation by dimerization: two sides to an incestuous relationship.  |  Jones, N. 1990. Cell. 61: 9-11. PMID: 2180585
6. Telomere length, c-myc and mad-1 expression could represent prognosis markers of myelodysplastic syndrome.  |  Poloni, A., et al. 2013. Leuk Res. 37: 1538-44. PMID: 24095110
7. A Bub1-Mad1 interaction targets the Mad1-Mad2 complex to unattached kinetochores to initiate the spindle checkpoint.  |  Moyle, MW., et al. 2014. J Cell Biol. 204: 647-57. PMID: 24567362
8. H19 Overexpression Induces Resistance to 1,25(OH)2D3 by Targeting VDR Through miR-675-5p in Colon Cancer Cells.  |  Chen, S., et al. 2017. Neoplasia. 19: 226-236. PMID: 28189050
9. The effectiveness of two types of MADS for OSA therapy.  |  Verburg, FE., et al. 2018. Clin Oral Investig. 22: 1995-2003. PMID: 29214378
10. Mad: a heterodimeric partner for Max that antagonizes Myc transcriptional activity.  |  Ayer, DE., et al. 1993. Cell. 72: 211-22. PMID: 8425218
11. Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites.  |  Zervos, AS., et al. 1993. Cell. 72: 223-32. PMID: 8425219
12. Oncogenic activity of the c-Myc protein requires dimerization with Max.  |  Amati, B., et al. 1993. Cell. 72: 233-45. PMID: 8425220

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mad 1 Antibody (F-1) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Mad 1 Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mad 1 Antibody (F-1) Specifications

Alternate Product Name: Mad 1

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ lambda light chain

Amino Acids: 1-221

Epitope Target: Mad 1

Epitope Species: human

Product Reactivity/Detection: Mad 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Mad 1 Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent