Mad 1 Antibody (F-1): sc-8012
- Mad 1 Antibody (F-1) is a mouse monoclonal IgG1 λ Mad 1 antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 1-221 representing full length Mad 1 of human origin
- Mad 1 Antibody (F-1) is recommended for detection of Mad 1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Mad 1 Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-8012 X, 200 µg/0.1 ml)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Mad 1 Antibody (F-1). This work is in progress.
QUICK LINKS
Mad 1 Antibody (F-1) is a mouse monoclonal IgG1 antibody that detects Mad 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Mad 1 (F-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Mad 1 protein plays a crucial role in the regulation of gene expression as a transcriptional repressor, particularly in cell differentiation and proliferation. Mad 1 protein functions by forming heterodimeric complexes with Max, which antagonize Myc-Max heterodimers′ activity, thereby inhibiting genes that promote cell growth and division. Mad 1 protein′s regulatory mechanism is vital for maintaining cellular homeostasis and preventing uncontrolled cell proliferation, which can lead to oncogenesis. Mad 1 protein′s stability, in contrast to highly regulated Myc protein, allows effective modulation of gene expression in response to various cellular signals. Understanding Mad 1 protein dynamics and interactions with other proteins is essential for elucidating complex networks that govern cellular behavior and developing potential therapeutic strategies targeting these pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Mad 1 Antibody (F-1) References:
- Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants. | Mukherjee, B., et al. 1992. Genes Dev. 6: 1480-92. PMID: 1644290
- Association of Myn, the murine homolog of max, with c-Myc stimulates methylation-sensitive DNA binding and ras cotransformation. | Prendergast, GC., et al. 1991. Cell. 65: 395-407. PMID: 1840505
- Intracellular leucine zipper interactions suggest c-Myc hetero-oligomerization. | Dang, CV., et al. 1991. Mol Cell Biol. 11: 954-62. PMID: 1990293
- Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc. | Blackwood, EM. and Eisenman, RN. 1991. Science. 251: 1211-7. PMID: 2006410
- Transcriptional regulation by dimerization: two sides to an incestuous relationship. | Jones, N. 1990. Cell. 61: 9-11. PMID: 2180585
- Telomere length, c-myc and mad-1 expression could represent prognosis markers of myelodysplastic syndrome. | Poloni, A., et al. 2013. Leuk Res. 37: 1538-44. PMID: 24095110
- A Bub1-Mad1 interaction targets the Mad1-Mad2 complex to unattached kinetochores to initiate the spindle checkpoint. | Moyle, MW., et al. 2014. J Cell Biol. 204: 647-57. PMID: 24567362
- H19 Overexpression Induces Resistance to 1,25(OH)2D3 by Targeting VDR Through miR-675-5p in Colon Cancer Cells. | Chen, S., et al. 2017. Neoplasia. 19: 226-236. PMID: 28189050
- The effectiveness of two types of MADS for OSA therapy. | Verburg, FE., et al. 2018. Clin Oral Investig. 22: 1995-2003. PMID: 29214378
- Mad: a heterodimeric partner for Max that antagonizes Myc transcriptional activity. | Ayer, DE., et al. 1993. Cell. 72: 211-22. PMID: 8425218
- Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites. | Zervos, AS., et al. 1993. Cell. 72: 223-32. PMID: 8425219
- Oncogenic activity of the c-Myc protein requires dimerization with Max. | Amati, B., et al. 1993. Cell. 72: 233-45. PMID: 8425220
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mad 1 Antibody (F-1) | sc-8012 | 200 µg/ml | $322.00 | |||
Mad 1 Antibody (F-1) X | sc-8012 X | 200 µg/0.1 ml | $322.00 | |||
Mad 1 Antibody (F-1) AC | sc-8012 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Mad 1 Antibody (F-1) HRP | sc-8012 HRP | 200 µg/ml | $322.00 | |||
Mad 1 Antibody (F-1) FITC | sc-8012 FITC | 200 µg/ml | $336.00 | |||
Mad 1 Antibody (F-1) PE | sc-8012 PE | 200 µg/ml | $349.00 | |||
Mad 1 Antibody (F-1) Alexa Fluor® 488 | sc-8012 AF488 | 200 µg/ml | $364.00 | |||
Mad 1 Antibody (F-1) Alexa Fluor® 546 | sc-8012 AF546 | 200 µg/ml | $364.00 | |||
Mad 1 Antibody (F-1) Alexa Fluor® 594 | sc-8012 AF594 | 200 µg/ml | $364.00 | |||
Mad 1 Antibody (F-1) Alexa Fluor® 647 | sc-8012 AF647 | 200 µg/ml | $364.00 | |||
Mad 1 Antibody (F-1) Alexa Fluor® 680 | sc-8012 AF680 | 200 µg/ml | $364.00 | |||
Mad 1 Antibody (F-1) Alexa Fluor® 790 | sc-8012 AF790 | 200 µg/ml | $364.00 |