mAChR M3 Double Nickase Plasmid (h): sc-400829-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- mAChR M3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- mAChR M3 Double Nickase Plasmid (h) and mAChR M3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CHRM3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: mAChR M3 Antibody (H-4): sc-518107
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
mAChR M3 Double Nickase Plasmid (h) | sc-400829-NIC | 20 µg | $410.00 | |||
mAChR M3 Double Nickase Plasmid (h2) | sc-400829-NIC-2 | 20 µg | $410.00 |
CHRM3 encodes the human muscarinic acetylcholine receptor M3 (mAChR M3), a G protein–coupled receptor that predominantly couples to Gq/11 to activate phospholipase Cβ, elevate intracellular Ca²⁺, and stimulate PKC-dependent signaling. Through PIP2 hydrolysis and downstream MAPK and transcriptional programs, mAChR M3 regulates smooth muscle contraction, glandular secretion, and neuronal excitability. Receptor activity integrates with calcium homeostasis, cytoskeletal remodeling, and cell-cycle–linked signaling networks that shape tissue physiology and stress responses. Altered CHRM3 signaling has been investigated in airway and gastrointestinal function, metabolic regulation, and proliferative signaling contexts relevant to oncology and neurobiology research.
mAChR M3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CHRM3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CHRM3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CHRM3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CHRM3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.