Each CRISPR/Cas9 Knockout (KO) Plasmid product consists of a pool of 3 plasmids designed to ensure identification and cleavage of a specific gene for maximum knockout efficiency. Each plasmid encodes a unique 20 nt sequence derived from the GeCKO (v2) library.
mAChR M2 CRISPR Plasmids (h)
- Target species: human
- CRISPR/Cas9 KO Plasmids consists of mAChR M2-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
- For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
- Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
- Gene knockdown or activation can be assayed using mAChR M2 Antibody (M2-2-B3): sc-33712
- All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
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CRISPR/Cas9 KO Plasmid
Double Nickase Plasmids
Recommended Control Products
SEE ALSO...
mAChR M2 Antibodies for analysis of cellular responses to mAChR M2 CRISPR Products
Learn more about the CRISPR System
CRISPR/Cas9 KO plasmids, Double Nickase Plasmids and CRISPR/dCas9 Activation plasmids are considered a "Licensed Product" and are to be used in accordance with the Limited/Label License. Click here for details
Description
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- mAChR M2 CRISPR/Cas9 Knockout (KO) Plasmid (h) consists of a pool of three plasmids each encoding the Cas9 nuclease and a mAChR M2-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency
- gRNA sequences are derived from the GeCKO (v2) library and direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
- **** NOTE: No corresponding HDR is available for this Knockout Plasmid. **** Please use sc-402394-KO-2 and sc-402394-HDR-2 if selection of cells using puromycin resistance is desired.
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: mAChR M2 Antibody (M2-2-B3): sc-33712
See Protocol Online.
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Description
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- mAChR M2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- The paired gRNAs encoded by mAChR M2 Double Nickase Plasmid (h2) differ from the paired gRNAs in mAChR M2 Double Nickase Plasmid (h)
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: mAChR M2 Antibody (M2-2-B3): sc-33712
See Protocol Online.
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Related Products
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
mAChR M2 CRISPR/Cas9 KO Plasmid (h) | sc-402394 | 20 µg | $397.00 | |||
mAChR M2 Double Nickase Plasmid (h) | sc-402394-NIC | 20 µg | $410.00 | |||
mAChR M2 Double Nickase Plasmid (h2) | sc-402394-NIC-2 | 20 µg | $410.00 |