Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Mac-2BP Double Nickase Plasmid (h): sc-403108-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mac-2BP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mac-2BP Double Nickase Plasmid (h) and Mac-2BP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LGALS3BP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mac-2BP Antibody (E-8): sc-374541
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mac-2BP Double Nickase Plasmid (h) | sc-403108-NIC | 20 µg | $410.00 | |||
Mac-2BP Double Nickase Plasmid (h2) | sc-403108-NIC-2 | 20 µg | $410.00 |
LGALS3BP encodes Mac-2BP (galectin-3–binding protein), a secreted and extracellular matrix–associated glycoprotein that modulates cell–cell and cell–matrix interactions through binding partners such as galectins and integrins. Mac-2BP is implicated in adhesion, migration, immune modulation, and remodeling of the extracellular milieu, with downstream effects on inflammatory signaling and tumor–stroma communication. Altered LGALS3BP expression has been reported across multiple cancer and fibrotic/inflammatory contexts and is frequently evaluated as a biomarker-linked component of disease-associated secretomes. These features make LGALS3BP a useful target for studying microenvironmental regulation, immune–epithelial crosstalk, and metastasis-relevant pathways in human cell models.
Mac-2BP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LGALS3BP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LGALS3BP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LGALS3BP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LGALS3BP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.