M33 Antibody (G-5): sc-515914
- M33 Antibody (G-5) is a mouse monoclonal IgM κ M33 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 17-44 near the N-terminus of M33 of human origin
- M33 Antibody (G-5) is recommended for detection of M33 of human origin by WB, IP, IF and ELISA
- Anti-M33 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for M33 Antibody (G-5). This work is in progress.
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SEE ALSO...
M33 Antibody (G-5) is a mouse monoclonal IgM kappa antibody that detects M33 by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). M33 (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. M33 , also known as CBX2 or MOD2, is a member of the Polycomb group (PcG) proteins, which are crucial for gene silencing and regulation of Hox genes through chromatin structure alteration during transcription. M33 plays a significant role in controlling retinoic acid response elements′ accessibility near Hox gene regulatory regions, utilizing both direct and indirect mechanisms. M33 interacts with other PcG proteins, such as MPc2 and MPc3, which share structural similarities and bind to PcG protein RING1 via a conserved c-box motif located at RING1′s C-terminus. M33 deficiency can lead to sex reversal by disrupting processes upstream of the Y-chromosome-specific SRY gene. M33 is implicated in various pathologies, including campomelic syndrome, an inherited disorder, and neoplastic conditions associated with allele loss in the corresponding genomic region. M33 (G-5) antibody′s versatility in multiple applications makes this reagent an essential tool for researchers studying gene regulation and associated disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
M33 Antibody (G-5) References:
- RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1. | García, E., et al. 1999. EMBO J. 18: 3404-18. PMID: 10369680
- HPC3 is a new human polycomb orthologue that interacts and associates with RING1 and Bmi1 and has transcriptional repression properties. | Bárdos, JI., et al. 2000. J Biol Chem. 275: 28785-92. PMID: 10825164
- Altered retinoic acid sensitivity and temporal expression of Hox genes in polycomb-M33-deficient mice. | Bel-Vialar, S., et al. 2000. Dev Biol. 224: 238-49. PMID: 10926763
- Activation of the beta globin locus by transcription factors and chromatin modifiers. | McMorrow, T., et al. 2000. EMBO J. 19: 4986-96. PMID: 10990462
- The polycomb protein MPc3 interacts with AF9, an MLL fusion partner in t(9;11)(p22;q23) acute leukemias. | Hemenway, CS., et al. 2001. Oncogene. 20: 3798-805. PMID: 11439343
- Efficacy and toxicity of the antimicrobial peptide M33 produced with different counter-ions. | Pini, A., et al. 2012. Amino Acids. 43: 467-73. PMID: 21984381
- Cytomegalovirus chemokine receptor M33 knockout reduces chronic allograft rejection in a murine aortic transplant model. | Fritz, NM., et al. 2021. Transpl Immunol. 64: 101359. PMID: 33301898
- M33 condenses chromatin through nuclear body formation and methylation of both histone H3 lysine 9 and lysine 27. | Lin, YR., et al. 2021. Biochim Biophys Acta Mol Cell Res. 1868: 119100. PMID: 34274396
- The Mouse Cytomegalovirus G Protein-Coupled Receptor Homolog, M33, Coordinates Key Features of In Vivo Infection via Distinct Components of Its Signaling Repertoire. | Ma, J., et al. 2022. J Virol. 96: e0186721. PMID: 34878888
- The CMV-encoded G protein-coupled receptors M33 and US28 play pleiotropic roles in immune evasion and alter host T cell responses. | White, TM., et al. 2022. Front Immunol. 13: 1047299. PMID: 36569845
- The Viral G-Protein-Coupled Receptor Homologs M33 and US28 Promote Cardiac Dysfunction during Murine Cytomegalovirus Infection. | Bonavita, CM., et al. 2023. Viruses. 15: PMID: 36992420
- Assignment of a Polycomb-like chromobox gene (CBX2) to human chromosome 17q25. | Gecz, J., et al. 1995. Genomics. 26: 130-3. PMID: 7782071
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
M33 Antibody (G-5) | sc-515914 | 200 µg/ml | $322.00 | |||
M33 Antibody (G-5) AC | sc-515914 AC | 500 µg/ml, 25% agarose | $424.00 | |||
M33 Antibody (G-5) HRP | sc-515914 HRP | 200 µg/ml | $322.00 | |||
M33 Antibody (G-5) FITC | sc-515914 FITC | 200 µg/ml | $336.00 | |||
M33 Antibody (G-5) PE | sc-515914 PE | 200 µg/ml | $349.00 | |||
M33 Antibody (G-5) Alexa Fluor® 488 | sc-515914 AF488 | 200 µg/ml | $364.00 | |||
M33 Antibody (G-5) Alexa Fluor® 546 | sc-515914 AF546 | 200 µg/ml | $364.00 | |||
M33 Antibody (G-5) Alexa Fluor® 594 | sc-515914 AF594 | 200 µg/ml | $364.00 | |||
M33 Antibody (G-5) Alexa Fluor® 647 | sc-515914 AF647 | 200 µg/ml | $364.00 | |||
M33 Antibody (G-5) Alexa Fluor® 680 | sc-515914 AF680 | 200 µg/ml | $364.00 | |||
M33 Antibody (G-5) Alexa Fluor® 790 | sc-515914 AF790 | 200 µg/ml | $364.00 |