Date published: 2026-11-9
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Tom20 (F-10): sc-17764. Western blot analysis of Tom20 expression in SK-N-MC (A) and HeLa (B) whole cell lysates. Detectio
Tom20 (F-10): sc-17764. Western blot analysis of Tom20 expression in SK-N-MC (A) and HeLa (B) whole cell lysates. Detection reagent used: m-IgGλ BP-HRP: sc-516132.
Tom20 (F-10): sc-17764. Western blot analysis of Tom20 expression in SK-N-MC (A) and HeLa (B) whole cell lysates. Detection reagent used: m-IgGλ BP-HRP: sc-516132.

m-IgGλ BP-HRP: sc-516132

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Datasheets
  • mouse IgG lambda binding protein (m-IgGλ BP) conjugated to Horseradish Peroxidase (HRP)
  • supplied at 200 µg in 0.5 ml volume
  • Highly recommended alternative to conventional anti-mouse IgG secondary antibodies for chemiluminescence Western blotting (WB [ECL]) and Immunohistochemistry (IHC)
  • Suitable for binding to most, but not all, mouse IgGλ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGκ light chain antibodies
  • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
  • For a Cruz Marker™ compatible mouse IgG lambda binding protein, use m-IgGλ BP-HRP (Cruz Marker) (sc-516132-CM) (Click here for datasheet)
  • For mouse IgGκ immunoglobulins we recommend m-IgGκ BP-HRP (sc-516102)
  • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
  • Also see our new m-IgGλ BP-FITC, PE, CFL 488, CFL 555, CFL 594, CFL 647, CFL 680 and CFL 790 fluorescent dye conjugates. These represent substantial improvements compared to conventional polyclonal anti-mouse secondary antibody fluorescent dye conjugates.
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Description
Datasheets and Protocols

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

Download thiomersal SDS

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

m-IgGλ BP-HRP

sc-516132
200 µg/0.5 ml
$93.00

m-IgGλ BP-HRP (Cruz Marker)

sc-516132-CM
200 µg/0.5 ml
$93.00

Do you store this Ab at 4C or -20C?

Asked by: Harley
Thank you for your question .Please store the antibody at 4C.
Answered by: BlakeJ
Date published: 2024-04-24

We didn't know our primary antibody subtype ( kappaor lamda), which 2nd we should order?

Asked by: yuhong
Thank you for your question. The significant majority of mouse monoclonal IgG primary antibodies (98%) have a kappa light chain. And therefore we would recommend using our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP): sc-516102 for Western blotting or immunohistochemistry. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp Only approximately 2% of mouse IgG contains a lambda light chain, but for those subtypes we do offer a lambda-specific mouse IgG binding protein, m-IgGλ BP-HRP: sc-516132.
Answered by: Technical Support
Date published: 2018-09-13

I was looking for an anti-rat secondary antibody to be used in WB, but I couldn´t find it. I need the secondary antibody for the primary antibodies: sc-58605, sc-58607, and sc-58596. Can you give me an alternative?

Asked by: Marcia1
Thank you for your question. Unfortunately, we do not currently offer any secondary antibodies specific for detection of rat immunoglobulins. We are working on developing new products, but these are not yet available. We recommend using any conventional anti-rat IgG secondary antibodies for detection of the primary antibodies you have referenced above.
Answered by: Technical Support
Date published: 2017-01-11
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Rated 5 out of 5 by SCBT QC Lab from Great for chemiluminescent western blotsThis detection reagent worked well for detecting Rho GDIα (C-2) antibody, sc-374579, in a chemiluminescent western blot assay. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by SCBT QC Lab from Great for chemiluminescent western blots!This detection reagent worked well for detecting ARP-1 (E-11) antibody, sc-271940, in a chemiluminescent western blot assay. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by erik999 from western blotWe have tested it in WB analysis and it works well. Recommend concentration 1:3000.
Date published: 2021-06-23
Rated 5 out of 5 by Anonymous from niceNot long ago, I bought this product, which was shipped in time and well packaged.It's suitable for my experiment. it's very helpful to carry out the experiment.
Date published: 2021-06-03
Rated 5 out of 5 by t888 from great as alternative for secondary antibodythis is the best alternative for serving as secondary antibody
Date published: 2020-12-01
Rated 4 out of 5 by Liu Liu from Western blottingUse 1:5000 dilution and works great for mouse liver lysates
Date published: 2018-01-15
Rated 4 out of 5 by Mukta S from Works wellThis antibody worked very well in several of my WB experiments.
Date published: 2017-12-19
Rated 5 out of 5 by chen Y from good result on WBThe m-IgGλ BP-HRP antibody works well in Werstern Blot detection using Rat articular chondrocytes cell at 1:800. we got a clear protein band.
Date published: 2017-07-14
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m-IgGλ BP-HRP is rated 4.5 out of 5 by 18.
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