Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LZTFL1 CRISPR/Cas9 KO Plasmid (m): sc-430035
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LZTFL1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LZTFL1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LZTFL1 Antibody (C-6): sc-376022
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LZTFL1 CRISPR/Cas9 KO Plasmid (m) | sc-430035 | 20 µg | $397.00 |
Overview
Lztfl1 encodes LZTFL1, a cytosolic protein implicated in regulation of primary cilium structure and trafficking, influencing compartmentalized signaling at the ciliary membrane. It has been linked to modulation of cilia-dependent pathways such as Hedgehog signaling and broader epithelial cell polarity and differentiation programs. Altered LZTFL1 expression or function has been associated with changes in cell migration and invasion phenotypes, supporting relevance to cancer biology and tissue homeostasis. In mouse models, Lztfl1 is used to investigate how ciliary signaling and epithelial organization shape developmental and disease-associated processes.
LZTFL1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lztfl1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lztfl1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lztfl1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LZTFL1 protein expression.
This CRISPR knockout system enables efficient generation of Lztfl1-deficient cell models for investigation of LZTFL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lztfl1 exon(s) critical for LZTFL1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lztfl1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LZTFL1 CRISPR/Cas9 KO Plasmid (m) and LZTFL1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lztfl1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LZTFL1 HDR Plasmid (m) and LZTFL1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lztfl1 homology arms to support homology-directed repair at defined Lztfl1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.