LXR beta/NER/NR1H2 CRISPR/Cas9 KO Plasmid (m): sc-423617

Overview

Nr1h2 encodes liver X receptor beta (LXRβ/NER), a ligand-activated nuclear receptor that functions as a transcriptional regulator of lipid and cholesterol homeostasis. In mouse cells, LXRβ forms heterodimers with RXR to modulate genes controlling reverse cholesterol transport, fatty acid metabolism, and inflammatory gene programs, integrating metabolic and innate immune signaling. This pathway intersects with macrophage polarization, adipocyte biology, and neuroinflammatory responses, linking Nr1h2 activity to mechanisms relevant to atherosclerosis, metabolic dysfunction, and CNS inflammatory phenotypes in experimental models. Because LXRβ influences transcriptional networks in multiple tissues, Nr1h2 perturbation is widely used to dissect nuclear receptor crosstalk and ligand-dependent gene regulation.

LXR beta/NER/NR1H2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nr1h2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nr1h2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nr1h2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LXR beta/NER/NR1H2 protein expression.

This CRISPR knockout system enables efficient generation of Nr1h2-deficient cell models for investigation of LXR beta/NER/NR1H2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Nr1h2 exon(s) critical for LXR beta/NER/NR1H2 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Nr1h2 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by LXR beta/NER/NR1H2 CRISPR/Cas9 KO Plasmid (m) and LXR beta/NER/NR1H2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nr1h2 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by LXR beta/NER/NR1H2 HDR Plasmid (m) and LXR beta/NER/NR1H2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nr1h2 homology arms to support homology-directed repair at defined Nr1h2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.