Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Lumican CRISPR/Cas9 KO Plasmid (h): sc-402001
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Lumican CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Lumican genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Lumican Antibody (B-9): sc-166871
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lumican CRISPR/Cas9 KO Plasmid (h) | sc-402001 | 20 µg | $397.00 |
Overview
LUM encodes lumican, a small leucine-rich proteoglycan of the extracellular matrix that binds collagen fibrils and regulates their assembly, spacing, and biomechanical properties. Through modulation of matrix organization and cell–matrix adhesion, lumican influences processes such as tissue remodeling, wound repair, and stromal signaling that intersect with pathways controlling migration, proliferation, and inflammatory responses. Altered LUM expression or extracellular distribution has been associated with fibrosis, corneal and connective tissue phenotypes, and tumor microenvironment remodeling, making it relevant to studies of matrix-driven disease mechanisms. Lumican is also used as a marker and functional node in research on stromal cell states and extracellular matrix homeostasis.
Lumican CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LUM gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LUM together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LUM open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Lumican protein expression.
This CRISPR knockout system enables efficient generation of LUM-deficient cell models for investigation of Lumican signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LUM exon(s) critical for Lumican function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LUM genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Lumican CRISPR/Cas9 KO Plasmid (h) and Lumican CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LUM locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Lumican HDR Plasmid (h) and Lumican HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LUM homology arms to support homology-directed repair at defined LUM target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.