LTA4H Double Nickase Plasmid (h): sc-404695-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LTA4H Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LTA4H Double Nickase Plasmid (h) and LTA4H Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LTA4H. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LTA4H Antibody (D-6): sc-390567
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LTA4H Double Nickase Plasmid (h) | sc-404695-NIC | 20 µg | $410.00 | |||
LTA4H Double Nickase Plasmid (h2) | sc-404695-NIC-2 | 20 µg | $410.00 |
Human LTA4H (leukotriene A4 hydrolase) is a bifunctional zinc metalloenzyme that converts leukotriene A4 to leukotriene B4, a potent lipid mediator that amplifies chemotaxis, cytokine signaling, and innate immune activation. Through the arachidonic acid and leukotriene biosynthesis pathway, LTA4H shapes inflammatory cell recruitment and contributes to the balance between pro-resolving and pro-inflammatory responses. Dysregulated LTA4H activity and LTB4 signaling have been implicated in chronic inflammatory conditions and immune-mediated tissue injury, and are frequently studied in the context of pulmonary, cardiovascular, and metabolic inflammation. As a cytosolic enzyme with broad effects on leukocyte behavior, LTA4H is a useful node for dissecting eicosanoid-driven signaling networks and inflammation-linked phenotypes in human cell models.
LTA4H Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LTA4H locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LTA4H. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LTA4H function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LTA4H-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.