Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LTβR Double Nickase Plasmid (m): sc-421483-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LTβR Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LTβR Double Nickase Plasmid (m) and LTβR Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ltbr. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LTβR Antibody (H-2): sc-398929
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LTβR Double Nickase Plasmid (m) | sc-421483-NIC | 20 µg | $410.00 |
Lymphotoxin beta receptor (LTβR), encoded by the mouse Ltbr gene, is a TNF receptor superfamily member that regulates lymphoid organ development and immune microenvironment organization. LTβR signaling engages canonical and noncanonical NF-κB pathways to control stromal cell activation, chemokine expression, and maintenance of lymphoid tissue architecture. Through these processes it influences leukocyte trafficking, antigen-presenting cell niches, and crosstalk between stromal and immune compartments. Altered LTβR activity has been linked to inflammatory and autoimmune phenotypes and contributes to mechanisms relevant to chronic inflammation and tumor–immune interactions.
LTβR Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ltbr locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ltbr. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ltbr function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ltbr-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.