LT-β Double Nickase Plasmid (h): sc-404663-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LT-β Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LT-β Double Nickase Plasmid (h) and LT-β Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LTB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LT-β Antibody (399CT9.3.4): sc-517341
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LT-β Double Nickase Plasmid (h) | sc-404663-NIC | 20 µg | $410.00 | |||
LT-β Double Nickase Plasmid (h2) | sc-404663-NIC-2 | 20 µg | $410.00 |
Lymphotoxin-β (LT-β), encoded by the human LTB gene, is a type II membrane protein of the TNF superfamily that assembles with lymphotoxin-α to form the LTα1β2 heterotrimer and signals primarily through the lymphotoxin-β receptor (LTβR). This axis activates canonical and noncanonical NF-κB pathways to regulate lymphoid organogenesis, stromal–immune crosstalk, chemokine networks, and maintenance of tissue microenvironments that support adaptive immunity. LTB contributes to organization of secondary lymphoid structures and mucosal immune responses, influencing antigen presentation niches and leukocyte trafficking programs. Dysregulated LTβR signaling has been implicated in chronic inflammation, autoimmune phenotypes, and tumor-associated immune remodeling, making LTB a relevant node for mechanistic studies in immunology and cancer biology.
LT-β Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LTB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LTB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LTB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LTB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.