LSm1 Antibody (A-9): sc-373685
- LSm1 Antibody (A-9) is a mouse monoclonal IgG1 κ LSm1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-133 representing full length LSm1 of human origin
- LSm1 Antibody (A-9) is recommended for detection of LSm1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
- Anti-LSm1 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-373685 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for LSm1 Antibody (A-9) for WB applications. These reagents are now offered in bundles with LSm1 Antibody (A-9) (see ordering information below).
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LSm1 Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects LSm1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LSm1 (A-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LSm1 protein plays a crucial role in RNA metabolism, forming heptameric complexes that are essential for proper assembly of ribosomal subunits. Specifically, LSm1 binds to the 3′-terminal U-tract of U6 small nuclear RNA, facilitating critical RNA-protein interactions and structural changes necessary for ribonucleoprotein complex formation. This function influences splicing efficiency and RNA molecule stability, which are vital processes in gene expression regulation. LSm1 is overexpressed in pancreatic cancer and certain breast cancer cell lines, while LSm1 downregulation has been implicated in prostate cancer progression, highlighting LSm1′s potential as a biomarker and therapeutic target in oncology. Human LSm1 is localized in cytoplasmic small, discrete foci, which also serve as localization sites for mRNA decapping enzyme Dcp1/2 and exonuclease Xm1, further emphasizing LSm1′s significance in RNA metabolism and cellular function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LSm1 Antibody (A-9) References:
- A Sm-like protein complex that participates in mRNA degradation. | Bouveret, E., et al. 2000. EMBO J. 19: 1661-71. PMID: 10747033
- Down-regulation of Lsm1 is involved in human prostate cancer progression. | Takahashi, S., et al. 2002. Br J Cancer. 86: 940-6. PMID: 11953827
- Lsm Proteins are required for normal processing and stability of ribosomal RNAs. | Kufel, J., et al. 2003. J Biol Chem. 278: 2147-56. PMID: 12438310
- Yeast Lsm1p-7p/Pat1p deadenylation-dependent mRNA-decapping factors are required for brome mosaic virus genomic RNA translation. | Noueiry, AO., et al. 2003. Mol Cell Biol. 23: 4094-106. PMID: 12773554
- Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54. | Chu, CY. and Rana, TM. 2006. PLoS Biol. 4: e210. PMID: 16756390
- Molecular and microscopic techniques for detection of Sarcocystis neurona sporocysts in fecal samples. | Elsheikha, HM., et al. 2006. J Egypt Soc Parasitol. 36: 713-25. PMID: 16927879
- Transforming function of the LSM1 oncogene in human breast cancers with the 8p11-12 amplicon. | Streicher, KL., et al. 2007. Oncogene. 26: 2104-14. PMID: 17001308
- Interactions between brome mosaic virus RNAs and cytoplasmic processing bodies. | Beckham, CJ., et al. 2007. J Virol. 81: 9759-68. PMID: 17609284
- The human LSm1-7 proteins colocalize with the mRNA-degrading enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci. | Ingelfinger, D., et al. 2002. RNA. 8: 1489-501. PMID: 12515382
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LSm1 Antibody (A-9) | sc-373685 | 200 µg/ml | $322.00 | |||
LSm1 Antibody (A-9): m-IgG Fc BP-HRP Bundle | sc-527406 | 200 µg Ab; 10 µg BP | $361.00 | |||
LSm1 Antibody (A-9): m-IgG1 BP-HRP Bundle | sc-532779 | 200 µg Ab; 20 µg BP | $361.00 | |||
LSm1 Antibody (A-9) X | sc-373685 X | 200 µg/0.1 ml | $322.00 | |||
LSm1 Antibody (A-9) AC | sc-373685 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LSm1 Antibody (A-9) HRP | sc-373685 HRP | 200 µg/ml | $322.00 | |||
LSm1 Antibody (A-9) FITC | sc-373685 FITC | 200 µg/ml | $336.00 | |||
LSm1 Antibody (A-9) PE | sc-373685 PE | 200 µg/ml | $349.00 | |||
LSm1 Antibody (A-9) Alexa Fluor® 488 | sc-373685 AF488 | 200 µg/ml | $364.00 | |||
LSm1 Antibody (A-9) Alexa Fluor® 546 | sc-373685 AF546 | 200 µg/ml | $364.00 | |||
LSm1 Antibody (A-9) Alexa Fluor® 594 | sc-373685 AF594 | 200 µg/ml | $364.00 | |||
LSm1 Antibody (A-9) Alexa Fluor® 647 | sc-373685 AF647 | 200 µg/ml | $364.00 | |||
LSm1 Antibody (A-9) Alexa Fluor® 680 | sc-373685 AF680 | 200 µg/ml | $364.00 | |||
LSm1 Antibody (A-9) Alexa Fluor® 790 | sc-373685 AF790 | 200 µg/ml | $364.00 |