Lsk/Ctk Antibody (H-1): sc-271174
- Lsk/Ctk Antibody (H-1) is a mouse monoclonal IgG1 κ Lsk/Ctk antibody provided at 200 µg/ml
- raised against amino acids 171-257 mapping within an internal region of Lsk of human origin
- Lsk/Ctk Antibody (H-1) is recommended for detection of Lsk of human origin and Ctk of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Lsk/Ctk Antibody (H-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Lsk/Ctk Antibody (H-1) for WB and IHC(P) applications. These reagents are now offered in bundles with Lsk/Ctk Antibody (H-1) (see ordering information below).
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Lsk/Ctk Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Lsk protein of human origin and the Ctk protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Lsk/Ctk (H-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Lsk and Ctk proteins are members of the Src family of tyrosine kinases, which play crucial roles in cellular signaling pathways that regulate cell growth, differentiation, and survival. These proteins modulate various cellular processes through phosphorylation, a post-translational modification that can activate or deactivate signaling cascades. Phosphorylation of tyrosine residues within these kinases leads to significant changes in activity and interactions with other proteins, influencing cellular responses to external stimuli. For example, phosphorylation of the carboxy terminal domain tyrosine, Y527, maintains the enzyme in an inactive state, while dephosphorylation allows for increased kinase activity. Understanding Lsk and Ctk dynamics, including regulatory mechanisms and interactions with other signaling proteins, remains essential for elucidating roles in health and disease, particularly in cancer and immune responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Lsk/Ctk Antibody (H-1) References:
- Cloning of a complementary DNA for a protein-tyrosine kinase that specifically phosphorylates a negative regulatory site of p60c-src. | Nada, S., et al. 1991. Nature. 351: 69-72. PMID: 1709258
- A protein tyrosine kinase involved in regulation of pp60c-src function. | Okada, M. and Nakagawa, H. 1989. J Biol Chem. 264: 20886-93. PMID: 2480346
- Ctk: a protein-tyrosine kinase related to Csk that defines an enzyme family. | Klages, S., et al. 1994. Proc Natl Acad Sci U S A. 91: 2597-601. PMID: 7511815
- Molecular cloning of lsk, a carboxyl-terminal src kinase (csk) related gene, expressed in leukocytes. | McVicar, DW., et al. 1994. Oncogene. 9: 2037-44. PMID: 7516063
- The when and how of Src regulation. | Cooper, JA. and Howell, B. 1993. Cell. 73: 1051-4. PMID: 7685656
- Disruption of the csk gene, encoding a negative regulator of Src family tyrosine kinases, leads to neural tube defects and embryonic lethality in mice. | Imamoto, A. and Soriano, P. 1993. Cell. 73: 1117-24. PMID: 7685657
- Csk inhibition of c-Src activity requires both the SH2 and SH3 domains of Src. | Superti-Furga, G., et al. 1993. EMBO J. 12: 2625-34. PMID: 7687537
- Ntk: a Csk-related protein-tyrosine kinase expressed in brain and T lymphocytes. | Chow, LM., et al. 1994. Proc Natl Acad Sci U S A. 91: 4975-9. PMID: 8197166
- Constitutive activation of Src family kinases in mouse embryos that lack Csk. | Nada, S., et al. 1993. Cell. 73: 1125-35. PMID: 8513497
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lsk/Ctk Antibody (H-1) | sc-271174 | 200 µg/ml | $322.00 | |||
Lsk/Ctk Antibody (H-1): m-IgGκ BP-HRP Bundle | sc-521865 | 200 µg Ab, 40 µg BP | $361.00 | |||
Lsk/Ctk Antibody (H-1): m-IgG1 BP-HRP Bundle | sc-533506 | 200 µg Ab; 20 µg BP | $361.00 | |||
Lsk/Ctk Antibody (H-1) AC | sc-271174 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Lsk/Ctk Antibody (H-1) HRP | sc-271174 HRP | 200 µg/ml | $322.00 | |||
Lsk/Ctk Antibody (H-1) FITC | sc-271174 FITC | 200 µg/ml | $336.00 | |||
Lsk/Ctk Antibody (H-1) PE | sc-271174 PE | 200 µg/ml | $349.00 | |||
Lsk/Ctk Antibody (H-1) Alexa Fluor® 488 | sc-271174 AF488 | 200 µg/ml | $364.00 | |||
Lsk/Ctk Antibody (H-1) Alexa Fluor® 546 | sc-271174 AF546 | 200 µg/ml | $364.00 | |||
Lsk/Ctk Antibody (H-1) Alexa Fluor® 594 | sc-271174 AF594 | 200 µg/ml | $364.00 | |||
Lsk/Ctk Antibody (H-1) Alexa Fluor® 647 | sc-271174 AF647 | 200 µg/ml | $364.00 | |||
Lsk/Ctk Antibody (H-1) Alexa Fluor® 680 | sc-271174 AF680 | 200 µg/ml | $364.00 | |||
Lsk/Ctk Antibody (H-1) Alexa Fluor® 790 | sc-271174 AF790 | 200 µg/ml | $364.00 |