Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Lsh Double Nickase Plasmid (h): sc-402466-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Lsh Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Lsh Double Nickase Plasmid (h) and Lsh Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HELLS. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Lsh Antibody (H-4): sc-46665
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lsh Double Nickase Plasmid (h) | sc-402466-NIC | 20 µg | $410.00 | |||
Lsh Double Nickase Plasmid (h2) | sc-402466-NIC-2 | 20 µg | $410.00 |
HELLS encodes Lsh (lymphoid-specific helicase), an SNF2-family ATP-dependent chromatin remodeler that supports DNA methylation maintenance and heterochromatin formation by facilitating access of DNMTs and other epigenetic regulators to nucleosomal DNA. Lsh contributes to replication-coupled chromatin assembly, transcriptional repression of repetitive elements, and preservation of genome stability through effects on chromatin compaction and DNA repair-associated processes. Dysregulation of HELLS has been linked to aberrant DNA methylation patterns, altered cell-cycle control, and genomic instability phenotypes observed across developmental disorders and cancer-associated epigenetic remodeling studies. As a nuclear chromatin factor, Lsh is frequently investigated in pathways connecting epigenetic inheritance, transposon silencing, and replication stress responses.
Lsh Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HELLS locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HELLS. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HELLS function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HELLS-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.